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Title: Evaluating the Genotoxicity of Cellulose Nanofibrils in a Co-Culture of Human Lung Epithelial Cells and Monocyte-derived Macrophages
Authors: Ventura, Célia 
Lourenço, Ana Filipa 
Sousa-Uva, António 
Ferreira, Paulo J.T. 
Silva, Maria João 
Keywords: Cellulose nanofibrils; Safety assessment; Immunotoxicity; Comet assay; Micronucleus assay
Issue Date: Apr-2018
Publisher: Elsevier
Citation: Ventura, C., Lourenço, A. F., Sousa-Uva, A. Ferreira, P. J., Silva, M.J. (2018). Evaluating the Genotoxicity of Cellulose Nanofibrils in a Co-Culture of Human Lung Epithelial Cells and Monocyte-derived Macrophages. Toxicology Letters. 10.1016/j.toxlet.2018.04.013
Abstract: Cellulose nanofibrils (CNF) are manufactured nanofibres that hold impressive expectations in forest, food, pharmaceutical, and biomedical industries. CNF production and applications are leading to an increased human exposure and thereby it is of utmost importance to assess its safety to health. In this study, we screened the cytotoxic, immunotoxic and genotoxic effects of a CNF produced by TEMPO-mediated oxidation of an industrial bleached Eucalyptus globulus kraft pulp on a co-culture of lung epithelial alveolar (A549) cells and monocyte-derived macrophages (THP-1 cells). The results indicated that low CNF concentrations can stimulate A549 cells proliferation, whereas higher concentrations are moderately toxic. Moreover, no proinflammatory cytokine IL- was detected in the co-culture medium suggesting no immunotoxicity. Although CNF treatment did not induce sizable levels of DNA damage in A549 cells, it leaded to micronuclei formation at 1.5 and 3 μg/cm2. These findings suggest that this type of CNF is genotoxic through aneugenic or clastogenic mechanisms. Noteworthy, cell overgrowth and genotoxicity, which are events relevant for cell malignant transformation, were observed at low CNF concentration levels, which are more realistic and relevant for human exposure, e.g., in occupational settings.
ISSN: 0378-4274
ESSN: 1879-3169
DOI: 10.1016/j.toxlet.2018.04.013
Rights: embargoedAccess
Appears in Collections:FCTUC Eng.Química - Artigos em Revistas Internacionais

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