Please use this identifier to cite or link to this item:
|Title:||Differential effects of p,p'-DDE on testis and liver mitochondria: implications for reproductive toxicology||Authors:||Mota, Paula C.
Pereira, Susana P.
Oliveira, Paulo J.
Moreno, António J.
|Keywords:||Action Potentials; Animals; Dichlorodiphenyl Dichloroethylene; Endpoint Determination; Energy Metabolism; Environmental Pollutants; Insecticides; Male; Membrane Potential, Mitochondrial; Mitochondria, Liver; Oxygen Consumption; Protons; Rats; Rats, Wistar; Testis||Issue Date:||Jan-2011||Serial title, monograph or event:||Reproductive toxicology (Elmsford, N.Y.)||Volume:||31||Issue:||1||Abstract:||The release of environmental contaminants can contribute to impaired male fertility. The bioenergetics of isolated liver mitochondria have been used as a toxicological indicator, an inexpensive first line model to screen possible effects of several substances. Here we report the effects of 2,2-bis(4-chlorophenyl)-1,1-dichloro-ethylene (DDE) on the bioenergetical parameters of testicular mitochondria. A significant decrease in repolarization potential (after a phosphorylative cycle), state 3 respiration and uncoupled respiration, with a concomitant increase in lag phase was found, demonstrating a decrease in mitochondrial function. Importantly, there was also a clear increase in maximum potential in DDE-treated testis mitochondria, which was not mirrored by more commonly used liver mitochondria. Indeed, comparative studies showed that testis and liver mitochondria have strikingly different sensitivities and patterns of response to DDE, indicating that testis mitochondria should be used as a primary toxicological model for a proper evaluation of putative effects of environmental toxicants on the bioenergetics of spermatogenesis and male fertility.||URI:||http://hdl.handle.net/10316/47553||DOI:||10.1016/j.reprotox.2010.09.010||Rights:||openAccess|
|Appears in Collections:||I&D CNC - Artigos em Revistas Internacionais|
Show full item record
Files in This Item:
|Mota et al._2010_Reproductive toxicology (Elmsford, N.Y.)_Differential effects of p,p'-DDE on testis and liver mitochondriaImplications-annotated.pdf||558.5 kB||Adobe PDF||View/Open|
checked on Nov 9, 2022
WEB OF SCIENCETM
checked on Aug 2, 2022
Page view(s) 50433
checked on Mar 20, 2023
checked on Mar 20, 2023
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.