Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/45103
Title: | Nuclear Magnetic Resonance (NMR) Study of the Effect of Cisplatin on the Metabolic Profile of MG-63 Osteosarcoma Cells | Authors: | Duarte, Iola F. Lamego, Inês Marques, Joana Marques, M. Paula M. Blaise, Benjamin J. Gil, Ana M. |
Keywords: | Alcohols; Amino Acids; Choline; Cisplatin; Humans; Kinetics; Lipids; Magnetic Resonance Spectroscopy; Metabolome; Osteosarcoma | Issue Date: | 2010 | Serial title, monograph or event: | Journal of Proteome Research | Volume: | 9 | Issue: | 11 | Abstract: | In the present study, (1)H HRMAS NMR spectroscopy was used to assess the changes in the intracellular metabolic profile of MG-63 human osteosarcoma (OS) cells induced by the chemotherapy agent cisplatin (CDDP) at different times of exposure. Multivariate analysis was applied to the cells spectra, enabling consistent variation patterns to be detected and drug-specific metabolic effects to be identified. Statistical recoupling of variables (SRV) analysis and spectral integration enabled the most relevant spectral changes to be evaluated, revealing significant time-dependent alterations in lipids, choline-containing compounds, some amino acids, polyalcohols, and nitrogenated bases. The metabolic relevance of these compounds in the response of MG-63 cells to CDDP treatment is discussed. | URI: | https://hdl.handle.net/10316/45103 | DOI: | 10.1021/pr100635n 10.1021/pr100635n |
Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
JPR_10_9.pdf | 798.24 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
37
checked on May 1, 2023
WEB OF SCIENCETM
Citations
5
37
checked on May 2, 2023
Page view(s) 50
574
checked on Oct 16, 2024
Download(s)
410
checked on Oct 16, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.