Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/45103
Title: Nuclear Magnetic Resonance (NMR) Study of the Effect of Cisplatin on the Metabolic Profile of MG-63 Osteosarcoma Cells
Authors: Duarte, Iola F. 
Lamego, Inês 
Marques, Joana 
Marques, M. Paula M. 
Blaise, Benjamin J. 
Gil, Ana M. 
Keywords: Alcohols; Amino Acids; Choline; Cisplatin; Humans; Kinetics; Lipids; Magnetic Resonance Spectroscopy; Metabolome; Osteosarcoma
Issue Date: 2010
Serial title, monograph or event: Journal of Proteome Research
Volume: 9
Issue: 11
Abstract: In the present study, (1)H HRMAS NMR spectroscopy was used to assess the changes in the intracellular metabolic profile of MG-63 human osteosarcoma (OS) cells induced by the chemotherapy agent cisplatin (CDDP) at different times of exposure. Multivariate analysis was applied to the cells spectra, enabling consistent variation patterns to be detected and drug-specific metabolic effects to be identified. Statistical recoupling of variables (SRV) analysis and spectral integration enabled the most relevant spectral changes to be evaluated, revealing significant time-dependent alterations in lipids, choline-containing compounds, some amino acids, polyalcohols, and nitrogenated bases. The metabolic relevance of these compounds in the response of MG-63 cells to CDDP treatment is discussed.
URI: http://hdl.handle.net/10316/45103
Other Identifiers: 10.1021/pr100635n
DOI: 10.1021/pr100635n
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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