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https://hdl.handle.net/10316/3886
Título: | Naloxone inhibits nicotine-induced receptor current and catecholamine secretion in bovine chromaffin cells | Autor: | Tomé, Ângelo R. Izaguirre, Victor Rosário, Luís M. Ceña, Valentín González-García, Carmen |
Palavras-chave: | Opioids; Nicotinic receptor; Currents; Catecholamine secretion; Patch-clamp | Data: | 2001 | Citação: | Brain Research. 903:1-2 (2001) 62-65 | Resumo: | Nicotine-induced catecholamine (CA) secretion and inward ionic currents were inhibited by the opioid antagonist naloxone in cultured bovine chromaffin cells. Naloxone inhibited nicotine-induced CA secretion, as detected by an on-line real-time electrochemical technique, in a dose-dependent manner (IC50=29 [mu]M). In voltage-clamped chromaffin cells, nicotine (10 [mu]M) evoked an average peak inward current of -146 pA that was inhibited by low concentrations of naloxone (42% at 0.1 [mu]M). The antagonist also inhibited total charge influx associated with nicotinic receptor activation (53% at 0.1 [mu]M). This provides strong evidence that naloxone modulation of nicotine-induced CA secretion does not involve opioid receptors but results from the direct interaction with the nicotinic receptor itself, which might also be the case for other related opioid compounds. | URI: | https://hdl.handle.net/10316/3886 | DOI: | 10.1016/S0006-8993(01)02388-5 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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filef62fb0ccd06b4f719e7db5f5ee217ed3.pdf | 75.36 kB | Adobe PDF | Ver/Abrir |
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