Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/3886
Title: | Naloxone inhibits nicotine-induced receptor current and catecholamine secretion in bovine chromaffin cells | Authors: | Tomé, Ângelo R. Izaguirre, Victor Rosário, Luís M. Ceña, Valentín González-García, Carmen |
Keywords: | Opioids; Nicotinic receptor; Currents; Catecholamine secretion; Patch-clamp | Issue Date: | 2001 | Citation: | Brain Research. 903:1-2 (2001) 62-65 | Abstract: | Nicotine-induced catecholamine (CA) secretion and inward ionic currents were inhibited by the opioid antagonist naloxone in cultured bovine chromaffin cells. Naloxone inhibited nicotine-induced CA secretion, as detected by an on-line real-time electrochemical technique, in a dose-dependent manner (IC50=29 [mu]M). In voltage-clamped chromaffin cells, nicotine (10 [mu]M) evoked an average peak inward current of -146 pA that was inhibited by low concentrations of naloxone (42% at 0.1 [mu]M). The antagonist also inhibited total charge influx associated with nicotinic receptor activation (53% at 0.1 [mu]M). This provides strong evidence that naloxone modulation of nicotine-induced CA secretion does not involve opioid receptors but results from the direct interaction with the nicotinic receptor itself, which might also be the case for other related opioid compounds. | URI: | https://hdl.handle.net/10316/3886 | DOI: | 10.1016/S0006-8993(01)02388-5 | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
filef62fb0ccd06b4f719e7db5f5ee217ed3.pdf | 75.36 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.