Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/27828
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dc.contributor.authorAttar, Aisha-
dc.contributor.authorGhica, M. Emilia-
dc.contributor.authorAmine, Aziz-
dc.contributor.authorBrett, Christopher M. A.-
dc.date.accessioned2014-12-09T14:18:39Z-
dc.date.available2014-12-09T14:18:39Z-
dc.date.issued2014-08-30-
dc.identifier.citationATTAR, Aisha [et. al] - Poly(neutral red) based hydrogen peroxide biosensor for chromium determination by inhibition measurements. "Journal of Hazardous Materials". ISSN 0304-3894. Vol. 279 (2014) p. 348–355por
dc.identifier.issn0304-3894-
dc.identifier.urihttp://hdl.handle.net/10316/27828-
dc.description.abstractAmperometric hydrogen peroxide enzyme inhibition biosensors based on horseradish peroxidase (HRP) immobilised on electropolymerised neutral red (NR) or directly on the surface of carbon film electrodes (CFE) have been successfully applied to the determination of toxic Cr(III) and Cr(VI). Parameters influencing the performance of the biosensor including the enzyme immobilisation method, the amount of hydrogen peroxide, applied potential and electrolyte pH were optimised. The inhibition of horseradish peroxidase by the chromium species was studied under the optimised conditions. Results from the quantitative analysis of chromium ions are discussed in terms of detection limit, linear range and sensitivity. The HRP kinetic interactions reveal mixed binding of Cr(III) with I50 = 3.8 μM and inhibition binding constant Ki = 11.3 μM at HRP/PNR/CFE biosensors and uncompetitive binding of Cr(VI) with I50 = 3.9 μM and Ki = 0.78 μM at HRP/CFE biosensors in the presence of H2O2 substrate. Interferences from other heavy metal ions were studied and the inhibition show very good selectivity towards Cr(III) and Cr(VI).por
dc.language.isoengpor
dc.publisherElsevierpor
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/230815/EU-
dc.rightsopenAccesspor
dc.subjectAmperometric biosensorpor
dc.subjectHorseradish peroxidasepor
dc.subjectPoly(neutral red)por
dc.subjectCr(III)por
dc.subjectCr(VI)por
dc.subjectEnzyme inhibitionpor
dc.titlePoly(neutral red) based hydrogen peroxide biosensor for chromium determination by inhibition measurementspor
dc.typearticlepor
degois.publication.firstPage348por
degois.publication.lastPage355por
degois.publication.titleJournal of Hazardous Materialspor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0304389414005846por
dc.peerreviewedYespor
dc.identifier.doi10.1016/j.jhazmat.2014.07.019-
degois.publication.volume279por
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
crisitem.author.researchunitCentre for Mechanical Engineering-
crisitem.author.orcid0000-0002-1590-0816-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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