Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/27352
Title: Development, optimization and application of an analytical methodology by ultra performance liquid chromatography–tandem mass spectrometry for determination of amanitins in urine and liver samples
Authors: Leite, Marta 
Freitas, Andreia 
Azul, Anabela Marisa 
Barbosa, Jorge 
Costa, Saul 
Ramos
Keywords: Amanitin; Tilmicosin; Urine; Liver
Issue Date: 17-Oct-2013
Publisher: Elsevier
Citation: LEITE, Marta [et. al] - Development, optimization and application of an analytical methodology by ultra performance liquid chromatography–tandem mass spectrometry for determination of amanitins in urine and liver samples. "Analytica Chimica Acta". ISSN 0003-2670. Vol. 799 (2013) p. 77-87
Abstract: Amanitins, highly toxic cyclopeptides isolated from various Amanita species, are the most potent poisons accounting for the hazardous effects on intestinal epithelium cells and hepatocytes, and probably the sole cause of fatal human poisoning. The present study was focused on the development, optimization and application of an analytical methodology by ultra performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS), following urine and liver sample preparation by protein precipitation with organic solvents, and solid phase extraction (SPE) procedure, for the determination of the amatoxins, α- and β-amanitin. Linearity, detection and quantification limits, selectivity, sensitivity, intra and inter-assay precision and recovery were studied, in order to guarantee reliability in the analytical results. The developed method proved to be specific and selective, with LOD (Limit of Detection) values for α- and β-amanitin of 0.22 and 0.20 ng mL−1 in urine and 10.9 and 9.7 ng g−1 in liver, respectively. LOQ (Limit of Quantification) values ranged from 0.46 to 0.57 ng mL−1in urine and 12.3–14.7 ng g−1 in tissue, for both amanitins. Linearity, in the range of 10.0–200.0 ng mL−1or ng g−1, shows that coefficients of correlation were greater than 0.997 for α-amanitin and 0.993 for β-amanitin. Precision was checked at three levels during three consecutive days with intra-day and inter-day coefficients of variation not greater than 15.2%. The extraction recovery presents good results for the concentrations analyzed, with values ranging from 90.2 to 112.9% for both matrices. Thus, the proposed analytical method is innovative, presents a high potential in the identification, detection and determination of α- and β-amanitins in urine and tissue samples, as well as in other biological samples, such as kidney and mushrooms.
URI: http://hdl.handle.net/10316/27352
ISSN: 0003-2670
DOI: 10.1016/j.aca.2013.08.044
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais
I&D CEFarmacêuticos - Artigos em Revistas Internacionais
I&D CFE - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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