Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/27260
DC FieldValueLanguage
dc.contributor.authorFrancisco, Vera-
dc.contributor.authorCosta, Gustavo-
dc.contributor.authorFigueirinha, Artur-
dc.contributor.authorMarques, Carla-
dc.contributor.authorPereira, Paulo-
dc.contributor.authorNeves, Bruno Miguel-
dc.contributor.authorLopes, Maria Celeste-
dc.contributor.authorGarcía-Rodríguez, Carmen-
dc.contributor.authorCruz, Maria Teresa-
dc.contributor.authorBatista, Maria Teresa-
dc.date.accessioned2014-10-14T10:47:33Z-
dc.date.available2014-10-14T10:47:33Z-
dc.date.issued2013-06-21-
dc.identifier.citationFRANCISCO, Vera [et. al] - Anti-inflammatory activity of Cymbopogon citratus leaves infusion via proteasome and nuclear factor-κB pathway inhibition: contribution of chlorogenic acid. "Journal of Ethnopharmacology". ISSN 0378-8741. Vol. 148 Nº. 1 (2013) p. 126-134por
dc.identifier.issn0378-8741-
dc.identifier.urihttps://hdl.handle.net/10316/27260-
dc.description.abstractEthnopharmacological relevance Cymbopogon citratus (DC.) Stapf leaves infusion is used in traditional medicine for the treatment of inflammatory conditions, however little is known about their bioactive compounds. Aim of the study Investigate the compounds responsible for anti-inflammatory potential of Cymbopogon citratus (Cy) on cytokines production induced by lipopolysaccharide (LPS) in human and mouse macrophages, and the action mechanisms involved. Materials and methods An essential oil-free infusion of Cy was prepared and polyphenol-rich fractions (PFs) were obtained from it by column chromatography. Chlorogenic acid (CGA) was identified, by HPLC/PDA/ESI-MSn. The expression of cytokines, namely TNF-α and CCL5, was analyzed by real-time RT-PCR, on LPS-stimulated human macrophages. Activation of nuclear factor (NF)-κB, a master regulator of inflammation, was investigated by western blot and gene reporter assay. Proteasome activity was assessed using a fluorogenic peptide. Results Cymbopogon citratus extract and its polyphenols inhibited the cytokine production on human macrophages. This supports the anti-inflammatory activity of Cy polyphenols in physiologically relevant cells. Concerning the effect on the activation of NF-κB pathway, the results pointed to an inhibition of LPS-induced NF-κB activation by Cy and PFs. CGA was identified, by HPLC/PDA/ESI-MSn, as the main phenolic acid of the Cy infusion, and it demonstrated to be, at least in part, responsible by that effect. Additionally, it was verified for the first time that Cy and PFs inhibited the proteasome activity, a complex that controls NF-κB activation, having CGA a strong contribution. Conclusions The results evidenced, for the first time, the anti-inflammatory properties of Cymbopogon citratus through proteasome inhibition and, consequently NF-κB pathway and cytokine expression. Additionally, Cy polyphenols, in particular chlorogenic acid, were highlighted as bioactive compounds.por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.subjectChlorogenic acidpor
dc.subjectCymbopogon citratuspor
dc.subjectCytokinespor
dc.subjectNF-κBpor
dc.subjectPolyphenolspor
dc.subjectProteasomepor
dc.titleAnti-inflammatory activity of Cymbopogon citratus leaves infusion via proteasome and nuclear factor-κB pathway inhibition: Contribution of chlorogenic acidpor
dc.typearticlepor
degois.publication.firstPage126por
degois.publication.lastPage134por
degois.publication.issue1por
degois.publication.titleJournal of Ethnopharmacologypor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S037887411300247Xpor
dc.peerreviewedYespor
dc.identifier.doi10.1016/j.jep.2013.03.077-
degois.publication.volume148por
item.cerifentitytypePublications-
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0003-3064-5718-
crisitem.author.orcid0000-0002-9908-2290-
crisitem.author.orcid0000-0002-6469-0894-
crisitem.author.orcid0000-0001-9846-6754-
crisitem.author.orcid0000-0003-4183-6202-
Appears in Collections:I&D CEFarmacêuticos - Artigos em Revistas Internacionais
FFUC- Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
I&D IBILI - Artigos em Revistas Internacionais
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