Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/27260
Título: | Anti-inflammatory activity of Cymbopogon citratus leaves infusion via proteasome and nuclear factor-κB pathway inhibition: Contribution of chlorogenic acid | Autor: | Francisco, Vera Costa, Gustavo Figueirinha, Artur Marques, Carla Pereira, Paulo Neves, Bruno Miguel Lopes, Maria Celeste García-Rodríguez, Carmen Cruz, Maria Teresa Batista, Maria Teresa |
Palavras-chave: | Chlorogenic acid; Cymbopogon citratus; Cytokines; NF-κB; Polyphenols; Proteasome | Data: | 21-Jun-2013 | Editora: | Elsevier | Citação: | FRANCISCO, Vera [et. al] - Anti-inflammatory activity of Cymbopogon citratus leaves infusion via proteasome and nuclear factor-κB pathway inhibition: contribution of chlorogenic acid. "Journal of Ethnopharmacology". ISSN 0378-8741. Vol. 148 Nº. 1 (2013) p. 126-134 | Título da revista, periódico, livro ou evento: | Journal of Ethnopharmacology | Volume: | 148 | Número: | 1 | Resumo: | Ethnopharmacological relevance Cymbopogon citratus (DC.) Stapf leaves infusion is used in traditional medicine for the treatment of inflammatory conditions, however little is known about their bioactive compounds. Aim of the study Investigate the compounds responsible for anti-inflammatory potential of Cymbopogon citratus (Cy) on cytokines production induced by lipopolysaccharide (LPS) in human and mouse macrophages, and the action mechanisms involved. Materials and methods An essential oil-free infusion of Cy was prepared and polyphenol-rich fractions (PFs) were obtained from it by column chromatography. Chlorogenic acid (CGA) was identified, by HPLC/PDA/ESI-MSn. The expression of cytokines, namely TNF-α and CCL5, was analyzed by real-time RT-PCR, on LPS-stimulated human macrophages. Activation of nuclear factor (NF)-κB, a master regulator of inflammation, was investigated by western blot and gene reporter assay. Proteasome activity was assessed using a fluorogenic peptide. Results Cymbopogon citratus extract and its polyphenols inhibited the cytokine production on human macrophages. This supports the anti-inflammatory activity of Cy polyphenols in physiologically relevant cells. Concerning the effect on the activation of NF-κB pathway, the results pointed to an inhibition of LPS-induced NF-κB activation by Cy and PFs. CGA was identified, by HPLC/PDA/ESI-MSn, as the main phenolic acid of the Cy infusion, and it demonstrated to be, at least in part, responsible by that effect. Additionally, it was verified for the first time that Cy and PFs inhibited the proteasome activity, a complex that controls NF-κB activation, having CGA a strong contribution. Conclusions The results evidenced, for the first time, the anti-inflammatory properties of Cymbopogon citratus through proteasome inhibition and, consequently NF-κB pathway and cytokine expression. Additionally, Cy polyphenols, in particular chlorogenic acid, were highlighted as bioactive compounds. | URI: | https://hdl.handle.net/10316/27260 | ISSN: | 0378-8741 | DOI: | 10.1016/j.jep.2013.03.077 | Direitos: | openAccess |
Aparece nas coleções: | I&D CEFarmacêuticos - Artigos em Revistas Internacionais FFUC- Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais I&D IBILI - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Anti-inflammatory activity of Cymbopogon citratus leaves infusion via proteasome.pdf | 702.05 kB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
90
Visto em 1/mai/2023
Citações WEB OF SCIENCETM
5
85
Visto em 2/ago/2024
Visualizações de página 50
561
Visto em 28/ago/2024
Downloads 20
1.439
Visto em 28/ago/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.