Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/25784
Title: | Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo | Authors: | Currais, Lara Loureiro, J. Santos, Conceição Canhoto, J. M. |
Keywords: | Cyphomandra betacea; Aneuploids; Mixoploids; Nuclear DNA content; Somatic embryogenesis | Issue Date: | 2013 | Publisher: | Springer Science | Serial title, monograph or event: | Plant Cell, Tissue and Organ Culture | Volume: | 114 | Issue: | 2 | Abstract: | Ploidy levels of short-term (1 and 2 years) and long-term (7 and 10 years) embryogenic cultures as well as of regenerated plantlets of tamarillo were analyzed by flow cytometry and chromosome counts. Embryogenic cultures were induced from expanding leaves cultured in the presence of Picloram or 2,4-dichlorophenoxyacetic acid (2,4-D) and monthly subcultured on the same media. Embryo development and plantlets were obtained following subculture of the embryogenic tissue in auxin free medium containing gibberellic acid (GA3). Seedlings and rooted shoots from axillary shoot proliferation were used as controls. The results showed that in long-term embryogenic cultures the ability to develop somatic embryos and plantlets was reduced. Embryogenic tissues maintained for 10 years were mostly aneuploids of the tetraploid (2n = 4x = 48) level whereas those kept in culture for 7 years or less were also mostly aneuploids but of the diploid (2n = 2x = 24) level. The results obtained by flow cytometry were, in general, consistent with those obtained by chromosome counts. The chromosome alteration observed in the embryogenic tissues was already present after 1 year of culture and increased with culture age, hence impairing the maintenance of these tissues for long periods without affecting chromosome stability of the regenerated plantlets. However, the occurrence of triploids and tetraploids as well as aneuploids can be useful for breeding purposes. A value around 23 pg/2C was found for the genome size of tamarillo largely exceeding the value previously published (15.50 pg/2C). | URI: | https://hdl.handle.net/10316/25784 | DOI: | 10.1007/s11240-013-0311-5 | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Plant Cell Tiss Organ Cult (2013) 114,149–159.pdf | 516.35 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
26
checked on May 1, 2023
WEB OF SCIENCETM
Citations
5
19
checked on May 2, 2023
Page view(s)
277
checked on Oct 16, 2024
Download(s)
354
checked on Oct 16, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.