Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/25573
Title: | Impact of anti-PLK1 siRNA-containing F3-targeted liposomes on the viability of both cancer and endothelial cells | Authors: | Gomes-da-Silva, Lígia C. Ramalho, José S. Lima, M. C. P. de Simões, Sérgio Moreira, João N. |
Keywords: | Nanotechnology; siRNA; PLK1; Gene silencing; Paclitaxel; Prostate cancer | Issue Date: | 2013 | Publisher: | Elsevier B.V. | Serial title, monograph or event: | European Journal of Pharmaceutics and Biopharmaceutics | Volume: | 85 | Issue: | 3 Part A | Abstract: | We have previously described the development of novel sterically stabilized F3-targeted pH-sensitive liposomes, which exhibited the ability to target both cancer and endothelial cells. Herein, the therapeutic potential of those liposomes was assessed upon encapsulation of a siRNA against a well-validated molecular target, PLK1. Treatment of prostate cancer (PC3) and angiogenic endothelial (HMEC-1) cells with F3-targeted liposomes containing anti-PLK1 siRNA resulted in a significant decrease in cell viability, which was mediated by a marked PLK1 silencing, both at the mRNA and protein levels. Furthermore, pre-treatment of PC3 cells with F3-targeted liposomes containing anti-PLK1 siRNA enabled a 3-fold reduction of paclitaxel IC50 and a 2.5-fold augment of the percentage of cancer cells in G2/mitosis arrest, which ultimately culminated in cell death. Overall, the F3-targeted nanocarrier containing an anti-PLK1 siRNA might constitute a valuable system for prostate cancer treatment, either applied in a single schedule or combined with conventional chemotherapy. | URI: | https://hdl.handle.net/10316/25573 | DOI: | 10.1016/j.ejpb.2013.04.007 | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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1-s2.0-S0939641113001537-main(1).pdf | 1.19 MB | Adobe PDF | View/Open |
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