Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/15687
Title: | Micropropagation of Melissa officinalis L. through proliferation of axillary shoots | Authors: | Tavares, A. C. Pimenta, M. C. Gonçalves, M. T. |
Keywords: | Micropropagação; Melissa officinalis | Issue Date: | 1996 | Publisher: | Springer - Verlag | Serial title, monograph or event: | Plant Cell Reports | Volume: | 15 | Abstract: | Multiple shoots were differentiated in cotyledonary nodes of 10 d old seedlings of Melissa officinalis, cultured on MS medium supplemented with BAP (0-4 mg/l). The production of shoots was further induced in subcultures of the original explant, after the first harvest of shoots (stumps), using similar conditions. The highest average number of shoots in the two inoculations was obtained with 2 mg/l of BAP: 24 axillary shoots per explant, 7 in the first inoculation and 17 in the second one. The maximum elongation of shoots was achieved with BAP at 0.2 mg/l, and higher concentrations of the hormone induced a decrease in their size. A range of BAP concentrations between 0.2 - 0.5 mg/l allowed the production of more shoots with a size suitable for rooting. Roots were induced in 30d old shoots, transferred to MS medium individually supplemented with IBA or NAA (0 - 4 mg/l). Micropropagated plants were successfully transferred to soil. | URI: | https://hdl.handle.net/10316/15687 | DOI: | 10.1007/BF00232072 | Rights: | openAccess |
Appears in Collections: | FCTUC Ciências da Terra - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
artigo melissa.pdf.pdf | 2.19 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
37
checked on Oct 14, 2024
Page view(s)
349
checked on Oct 15, 2024
Download(s)
424
checked on Oct 15, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.