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Title: Towards the Maturation and Characterization of Smooth Muscle Cells Derived from Human Embryonic Stem Cells
Authors: Vazão, Helena 
Neves, Ricardo Pires das 
Grãos, Mário 
Ferreira, Lino 
Issue Date: 10-Mar-2011
Publisher: Public Library of Science
Citation: VAZÃO, Helena - Towards the Maturation and Characterization of Smooth Muscle Cells Derived from Human Embryonic Stem Cells. "PLoS ONE". ISSN 1932-6203. 6:3 (2011) e17771
Serial title, monograph or event: PLoS ONE
Volume: 6
Issue: 3
Abstract: In this study we demonstrate that CD34+ cells derived from human embryonic stem cells (hESCs) have higher smooth muscle cell (SMC) potential than CD34− cells. We report that from all inductive signals tested, retinoic acid (RA) and platelet derived growth factor (PDGFBB) are the most effective agents in guiding the differentiation of CD34+ cells into smooth muscle progenitor cells (SMPCs) characterized by the expression of SMC genes and proteins, secretion of SMC-related cytokines, contraction in response to depolarization agents and vasoactive peptides and expression of SMC-related genes in a 3D environment. These cells are also characterized by a low organization of the contractile proteins and the contractility response is mediated by Ca2+, which involves the activation of Rho A/Rho kinase- and Ca2+/calmodulin (CaM)/myosin light chain kinase (MLCK)-dependent pathways. We further show that SMPCs obtained from the differentiation of CD34+ cells with RA, but not with PDGFBB, can be maturated in medium supplemented with endothelin-1 showing at the end individualized contractile filaments. Overall the hESC-derived SMCs presented in this work might be an unlimited source of SMCs for tissue engineering and regenerative medicine.
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0017771
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

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