Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/15680
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Vazão, Helena | - |
dc.contributor.author | Neves, Ricardo Pires das | - |
dc.contributor.author | Grãos, Mário | - |
dc.contributor.author | Ferreira, Lino | - |
dc.date.accessioned | 2011-08-12T15:28:57Z | - |
dc.date.available | 2011-08-12T15:28:57Z | - |
dc.date.issued | 2011-03-10 | - |
dc.identifier.citation | VAZÃO, Helena - Towards the Maturation and Characterization of Smooth Muscle Cells Derived from Human Embryonic Stem Cells. "PLoS ONE". ISSN 1932-6203. 6:3 (2011) e17771 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://hdl.handle.net/10316/15680 | - |
dc.description.abstract | In this study we demonstrate that CD34+ cells derived from human embryonic stem cells (hESCs) have higher smooth muscle cell (SMC) potential than CD34− cells. We report that from all inductive signals tested, retinoic acid (RA) and platelet derived growth factor (PDGFBB) are the most effective agents in guiding the differentiation of CD34+ cells into smooth muscle progenitor cells (SMPCs) characterized by the expression of SMC genes and proteins, secretion of SMC-related cytokines, contraction in response to depolarization agents and vasoactive peptides and expression of SMC-related genes in a 3D environment. These cells are also characterized by a low organization of the contractile proteins and the contractility response is mediated by Ca2+, which involves the activation of Rho A/Rho kinase- and Ca2+/calmodulin (CaM)/myosin light chain kinase (MLCK)-dependent pathways. We further show that SMPCs obtained from the differentiation of CD34+ cells with RA, but not with PDGFBB, can be maturated in medium supplemented with endothelin-1 showing at the end individualized contractile filaments. Overall the hESC-derived SMCs presented in this work might be an unlimited source of SMCs for tissue engineering and regenerative medicine. | por |
dc.description.sponsorship | Marie Curie-Reintegration Grant | por |
dc.description.sponsorship | MIT-Portugal program | - |
dc.description.sponsorship | Crioestaminal | - |
dc.description.sponsorship | Associação Viver a Ciência | - |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (PTDC/SA-BEB/098468/2008 and PTDC/CTM/099659/2008 to L.F.; SFRH/BD/40077/2007 to H.V.) | - |
dc.language.iso | eng | por |
dc.publisher | Public Library of Science | por |
dc.rights | openAccess | por |
dc.title | Towards the Maturation and Characterization of Smooth Muscle Cells Derived from Human Embryonic Stem Cells | por |
dc.type | article | por |
degois.publication.firstPage | e17771 | por |
degois.publication.issue | 3 | por |
degois.publication.title | PLoS ONE | por |
dc.peerreviewed | Yes | por |
dc.identifier.doi | 10.1371/journal.pone.0017771 | - |
degois.publication.volume | 6 | por |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-5955-4804 | - |
crisitem.author.orcid | 0000-0002-2707-1488 | - |
crisitem.author.orcid | 0000-0001-8985-9302 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Towards the Maturation and Characterization of Smooth Muscle Cells.pdf | 1.34 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
31
checked on Aug 26, 2024
WEB OF SCIENCETM
Citations
5
27
checked on Aug 2, 2024
Page view(s) 5
1,207
checked on Aug 27, 2024
Download(s) 50
483
checked on Aug 27, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.