Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/13014
Title: Cell-Penetrating Peptides - Mechanisms of Cellular Uptake and Generation of Delivery Systems
Authors: Trabulo, Sara 
Cardoso, Ana Luísa 
Mano, Miguel 
Lima, Maria C. Pedroso de 
Keywords: Cell-penetrating peptides; Nucleic acid delivery; Non-viral vectors
Issue Date: 30-Mar-2010
Publisher: Molecular Diversity Preservation International
Keywords: Cell-penetrating peptides; Nucleic acid delivery; Non-viral vectors
Issue Date: 30-Mar-2010
Publisher: Molecular Diversity Preservation International
Citation: Pharmaceuticals. 3:4 (2010) 961-993
Abstract: The successful clinical application of nucleic acid-based therapeutic strategies has been limited by the poor delivery efficiency achieved by existing vectors. The development of alternative delivery systems for improved biological activity is, therefore, mandatory. Since the seminal observations two decades ago that the Tat protein, and derived peptides, can translocate across biological membranes, cell-penetrating peptides (CPPs) have been considered one of the most promising tools to improve non-invasive cellular delivery of therapeutic molecules. Despite extensive research on the use of CPPs for this purpose, the exact mechanisms underlying their cellular uptake and that of peptide conjugates remain controversial. Over the last years, our research group has been focused on the S413-PV cell-penetrating peptide, a prototype of this class of peptides that results from the combination of 13-amino-acid cell penetrating sequence derived from the Dermaseptin S4 peptide with the SV40 large T antigen nuclear localization signal. By performing an extensive biophysical and biochemical characterization of this peptide and its analogs, we have gained important insights into the mechanisms governing the interaction of CPPs with cells and their translocation across biological membranes. More recently, we have started to explore this peptide for the intracellular delivery of nucleic acids (plasmid DNA, siRNA and oligonucleotides). In this review we discuss the current knowledge of the mechanisms responsible for the cellular uptake of cell-penetrating peptides, including the S413-PV peptide, and the potential of peptide-based formulations to mediate nucleic acid delivery
URI: http://hdl.handle.net/10316/13014
ISSN: 1424-8247
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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