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Title: Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula
Authors: Silva, G. J. da 
Quinteira, S. 
Bértolo, E. 
Sousa, J. C. 
Gallego, L. 
Duarte, A. 
Peixe, L. 
Keywords: Acinetobacter spp.; Epidemic clones; Oxacillinases; B-lactamases
Issue Date: Jul-2004
Publisher: Oxford University Press
Citation: Journal of Antimicrobial Chemotherapy. 54:1 (2004) 255–258
Abstract: Objective: The main objectives of this study were to assess the clonal relatedness of Acinetobacter baumannii carbapenem-resistant isolates recovered from the Iberian Peninsula and to investigate the production of carbapenemases. Methods: One hundred and sixty-two imipenem-resistant A. baumannii isolates were collected from 1998 to 2003 in three Portuguese university hospitals. An imipenem-resistant isolate (988FFP strain) recovered in 1995 from a smaller hospital unit, was also included, as well as an OXA-40-producing A. baumannii Spanish strain (SM28). Susceptibility tests were carried out by disc diffusion and Etest methods. DNA fingerprints were obtained by PFGE of Apa I-digested chromosomal DNA. Carbapene- mase activity was determined by a bioassay and spectrophotometry. The detection of the blaOXA-40 gene was conducted through PCR analysis, cloning and nucleotide sequencing. Results: All the isolates presented a similar multi-resistance pattern, including imipenem (MIC >32 mg/L). The Iberian isolates showed an identical PFGE pattern with minor band variations, including isolate 988FFP collected in 1995. PCR results revealed a blaOXA-type gene in 65 isolates and nucleotide sequence analysis revealed the presence of the blaOXA-40 gene in seven representative Portuguese iso- lates from the various geographically dispersed hospitals. Conclusions: Our results indicate that a multi-resistant epidemic clone of A. baumannii, carrying blaOXA-40, is disseminated in the Iberian Peninsula, persisting in Portugal since 1995
ISSN: 0305-7453
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais

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