Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/12635
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dc.contributor.authorSilva, G. J. da-
dc.contributor.authorQuinteira, S.-
dc.contributor.authorBértolo, E.-
dc.contributor.authorSousa, J. C.-
dc.contributor.authorGallego, L.-
dc.contributor.authorDuarte, A.-
dc.contributor.authorPeixe, L.-
dc.date.accessioned2010-03-01T15:12:30Z-
dc.date.available2010-03-01T15:12:30Z-
dc.date.issued2004-07-
dc.identifier.citationJournal of Antimicrobial Chemotherapy. 54:1 (2004) 255–258en_US
dc.identifier.issn0305-7453-
dc.identifier.urihttps://hdl.handle.net/10316/12635-
dc.description.abstractObjective: The main objectives of this study were to assess the clonal relatedness of Acinetobacter baumannii carbapenem-resistant isolates recovered from the Iberian Peninsula and to investigate the production of carbapenemases. Methods: One hundred and sixty-two imipenem-resistant A. baumannii isolates were collected from 1998 to 2003 in three Portuguese university hospitals. An imipenem-resistant isolate (988FFP strain) recovered in 1995 from a smaller hospital unit, was also included, as well as an OXA-40-producing A. baumannii Spanish strain (SM28). Susceptibility tests were carried out by disc diffusion and Etest methods. DNA fingerprints were obtained by PFGE of Apa I-digested chromosomal DNA. Carbapene- mase activity was determined by a bioassay and spectrophotometry. The detection of the blaOXA-40 gene was conducted through PCR analysis, cloning and nucleotide sequencing. Results: All the isolates presented a similar multi-resistance pattern, including imipenem (MIC >32 mg/L). The Iberian isolates showed an identical PFGE pattern with minor band variations, including isolate 988FFP collected in 1995. PCR results revealed a blaOXA-type gene in 65 isolates and nucleotide sequence analysis revealed the presence of the blaOXA-40 gene in seven representative Portuguese iso- lates from the various geographically dispersed hospitals. Conclusions: Our results indicate that a multi-resistant epidemic clone of A. baumannii, carrying blaOXA-40, is disseminated in the Iberian Peninsula, persisting in Portugal since 1995en_US
dc.language.isoengen_US
dc.publisherOxford University Pressen_US
dc.rightsopenAccessen_US
dc.subjectAcinetobacter spp.en_US
dc.subjectEpidemic clonesen_US
dc.subjectOxacillinasesen_US
dc.subjectB-lactamasesen_US
dc.titleLong-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsulaen_US
dc.typearticleen_US
dc.identifier.doi10.1093/jac/dkh269-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.fulltextCom Texto completo-
item.languageiso639-1en-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-7479-8540-
Appears in Collections:FFUC- Artigos em Revistas Internacionais
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