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Title: Hypothermia Does Not Boost the Neuroprotection Promoted by Umbilical Cord Blood Cells in a Neonatal Hypoxia-Ischemia Rat Model
Authors: Serrenho, Inês 
Cardoso, Carla M.
Grãos, Mário 
Dinis, Alexandra
Manadas, Bruno 
Baltazar, Graça 
Keywords: neonatal hypoxic-ischemic encephalopathy; umbilical cord blood cells; cell therapy; therapeutic hypothermia; neonatal brain injury
Issue Date: 23-Dec-2022
Publisher: MDPI
Project: This work was financed by the European Regional Development Fund (ERDF), through the COMPETE 2020-Operational Programme for Competitiveness and Internationalisation and Portuguese national funds via FCT-Fundação para a Ciência e a Tecnologia, under projects POCI- 01–0145-FEDER-029311 and POCI-01-0247-FEDER-045311; UIDB/04539/2020, UIDP/04539/2020, LA/P/0058/2020, UID/Multi/00709/2019, and UIDB/00709/2020; and by funds to the PPBIPortuguese Platform of Bio Imaging through the Project POCI-01-0145-FEDER-022122. Inês Serrenho acknowledges the Ph.D. incentive fellowship financed by Santander Totta (BID/FCS/2020) and the Ph.D. individual fellowship financed by FCT—Fundação para a Ciência e a Tecnologia (2021.07854.BD). 
Serial title, monograph or event: International Journal of Molecular Sciences
Volume: 24
Issue: 1
Abstract: Neonatal hypoxic-ischemic encephalopathy (HIE) is one of the leading causes of death and long-term disability in the perinatal period. Currently, therapeutic hypothermia is the standard of care for this condition with modest efficacy and strict enrollment criteria. Therapy with umbilical cord blood cells (UCBC) has come forward as a strong candidate for the treatment of neonatal HIE, but no preclinical studies have yet compared the action of UCBC combined with hypothermia (HT) with the action of each therapy by itself. Thus, to evaluate the potential of each therapeutic approach, a hypoxic-ischemic brain lesion was induced in postnatal day ten rat pups; two hours later, HT was applied for 4 h; and 24, 48, and 72 h post-injury, UCBC were administered intravenously. The neonatal hypoxic-ischemic injury led to a brain lesion involving about 48% of the left hemisphere that was not improved by HT (36%) or UCBC alone (28%), but only with the combined therapies (25%; p = 0.0294). Moreover, a decrease in glial reactivity and improved functional outcomes were observed in both groups treated with UCBC. Overall, these results support UCBC as a successful therapeutic approach for HIE, even when treatment with therapeutic hypothermia is not possible.
ISSN: 1422-0067
DOI: 10.3390/ijms24010257
Rights: openAccess
Appears in Collections:IIIUC - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais

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