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Title: | TRAP1 Is Expressed in Human Retinal Pigment Epithelial Cells and Is Required to Maintain their Energetic Status | Authors: | Rego, Inês Ramos Silvério, Daniela Eufrásio, Maria Isabel Pinhanços, Sandra Sofia Lopes da Costa, Bruna Teixeira, José Fernandes, Hugo Kong, Yang Li, Yao Tsang, Stephen H Oliveira, Paulo J. Fernandes, Rosa Quinn, Peter M. J. Santos, P. F. Ambrósio, António Francisco Alves, C. Henrique |
Keywords: | age-relatedmacular degeneration (AMD); retinal pigment epithelium(RPE); mitochondria; oxidative stress; tumor necrosis factor receptor-associated protein 1 (TRAP1) | Issue Date: | 4-Feb-2023 | Publisher: | MDPI | Project: | This research was funded by National Funds via FCT (Foundation for Science and Technology), through the Strategic Project UIDB/04539/2020 and UIDP/04539/2020 (CIBB), and Centro 2020 Regional Operational Programme (CENTRO-01-0145-FEDER-000008: BrainHealth 2020) to A.F.A., CEECIND/00886/2017 to C.H.A., CEECIND/01880/2018 to H.F. and the project ResetAgeing (reference 952266) and BIO-MED (CENTRO-01-0145-FEDER-181228 co-financed by the European Regional Development Fund (FEDER) through the Center Regional Operational Program (CENTRO 2020), from Portugal 2020) to H.F. P.M.J.Q. is the current recipient of a Curing Retinal Blindness Foundation grant, a Knights Templar Eye Foundation Career Starter grant, an Uplifting Athletes Young Investigator grant, the International Retinal Research Foundation Loris and David Rich Postdoctoral Scholar Award, and a New York Stem Cell Foundation–Druckenmiller Fellowship. S.H.T. and The Jonas Children’s Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory are supported by the National Institutes of Health [P30EY019007, R01EY018213, R01EY024698, R01EY026682, R24EY027285, U01EY030580], National Cancer Institute Core [5P30CA013696], Foundation Fighting Blindness [TA-NMT-0116-0692-COLU], and the Research to Prevent Blindness (RPB) Physician-Scientist Award. B.L.D.C. is a recipient of the Capes PhD scholarship. Images taken on the Nikon Ti Eclipse inverted microscope were performed at the Confocal and Specialized Microscopy Shared Resource of the Herbert Irving Comprehensive Cancer Center at Columbia University, supported by NIH grant #P30 CA013696 (National Cancer Institute). | Serial title, monograph or event: | Antioxidants | Volume: | 12 | Issue: | 2 | Abstract: | Age-related macular degeneration (AMD) is the leading cause of severe vision loss and blindness in elderly people worldwide. The damage to the retinal pigment epithelium (RPE) triggered by oxidative stress plays a central role in the onset and progression of AMD and results from the excessive accumulation of reactive oxygen species (ROS) produced mainly by mitochondria. Tumor necrosis factor receptor-associated protein 1 (TRAP1) is a mitochondrial molecular chaperone that contributes to the maintenance of mitochondrial integrity by decreasing the production and accumulation of ROS. The present study aimed to evaluate the presence and the role of TRAP1 in the RPE. Here, we report that TRAP1 is expressed in human adult retinal pigment epithelial cells and is located mainly in the mitochondria. Exposure of RPE cells to hydrogen peroxide decreases the levels of TRAP1. Furthermore, TRAP1 silencing increases intracellular ROS production and decreases mitochondrial respiratory capacity without affecting cell proliferation. Together, these findings offer novel insights into TRAP1 functions in RPE cells, opening possibilities to develop new treatment options for AMD. | URI: | https://hdl.handle.net/10316/113949 | ISSN: | 2076-3921 | DOI: | 10.3390/antiox12020381 | Rights: | openAccess |
Appears in Collections: | I&D CIBB - Artigos em Revistas Internacionais FCTUC Ciências da Vida - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais |
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