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Title: TRAP1 Is Expressed in Human Retinal Pigment Epithelial Cells and Is Required to Maintain their Energetic Status
Authors: Rego, Inês Ramos 
Silvério, Daniela 
Eufrásio, Maria Isabel 
Pinhanços, Sandra Sofia 
Lopes da Costa, Bruna
Teixeira, José 
Fernandes, Hugo
Kong, Yang
Li, Yao
Tsang, Stephen H
Oliveira, Paulo J. 
Fernandes, Rosa 
Quinn, Peter M. J.
Santos, P. F. 
Ambrósio, António Francisco 
Alves, C. Henrique 
Keywords: age-relatedmacular degeneration (AMD); retinal pigment epithelium(RPE); mitochondria; oxidative stress; tumor necrosis factor receptor-associated protein 1 (TRAP1)
Issue Date: 4-Feb-2023
Publisher: MDPI
Project: This research was funded by National Funds via FCT (Foundation for Science and Technology), through the Strategic Project UIDB/04539/2020 and UIDP/04539/2020 (CIBB), and Centro 2020 Regional Operational Programme (CENTRO-01-0145-FEDER-000008: BrainHealth 2020) to A.F.A., CEECIND/00886/2017 to C.H.A., CEECIND/01880/2018 to H.F. and the project ResetAgeing (reference 952266) and BIO-MED (CENTRO-01-0145-FEDER-181228 co-financed by the European Regional Development Fund (FEDER) through the Center Regional Operational Program (CENTRO 2020), from Portugal 2020) to H.F. P.M.J.Q. is the current recipient of a Curing Retinal Blindness Foundation grant, a Knights Templar Eye Foundation Career Starter grant, an Uplifting Athletes Young Investigator grant, the International Retinal Research Foundation Loris and David Rich Postdoctoral Scholar Award, and a New York Stem Cell Foundation–Druckenmiller Fellowship. S.H.T. and The Jonas Children’s Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory are supported by the National Institutes of Health [P30EY019007, R01EY018213, R01EY024698, R01EY026682, R24EY027285, U01EY030580], National Cancer Institute Core [5P30CA013696], Foundation Fighting Blindness [TA-NMT-0116-0692-COLU], and the Research to Prevent Blindness (RPB) Physician-Scientist Award. B.L.D.C. is a recipient of the Capes PhD scholarship. Images taken on the Nikon Ti Eclipse inverted microscope were performed at the Confocal and Specialized Microscopy Shared Resource of the Herbert Irving Comprehensive Cancer Center at Columbia University, supported by NIH grant #P30 CA013696 (National Cancer Institute). 
Serial title, monograph or event: Antioxidants
Volume: 12
Issue: 2
Abstract: Age-related macular degeneration (AMD) is the leading cause of severe vision loss and blindness in elderly people worldwide. The damage to the retinal pigment epithelium (RPE) triggered by oxidative stress plays a central role in the onset and progression of AMD and results from the excessive accumulation of reactive oxygen species (ROS) produced mainly by mitochondria. Tumor necrosis factor receptor-associated protein 1 (TRAP1) is a mitochondrial molecular chaperone that contributes to the maintenance of mitochondrial integrity by decreasing the production and accumulation of ROS. The present study aimed to evaluate the presence and the role of TRAP1 in the RPE. Here, we report that TRAP1 is expressed in human adult retinal pigment epithelial cells and is located mainly in the mitochondria. Exposure of RPE cells to hydrogen peroxide decreases the levels of TRAP1. Furthermore, TRAP1 silencing increases intracellular ROS production and decreases mitochondrial respiratory capacity without affecting cell proliferation. Together, these findings offer novel insights into TRAP1 functions in RPE cells, opening possibilities to develop new treatment options for AMD.
ISSN: 2076-3921
DOI: 10.3390/antiox12020381
Rights: openAccess
Appears in Collections:I&D CIBB - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
I&D ICBR - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais

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