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https://hdl.handle.net/10316/112596
Title: | Restoring tumor immunogenicity with dendritic cell reprogramming | Authors: | Zimmermannova, Olga Ferreira, Alexandra G. Ascic, Ervin Velasco Santiago, Marta Kurochkin, Ilia Hansen, Morten Met, Özcan Caiado, Inês Shapiro, Ilja E. Michaux, Justine Humbert, Marion Soto-Cabrera, Diego Benonisson, Hreinn Silvério-Alves, Rita Gomez-Jimenez, David Bernardo, Carina Bauden, Monika Andersson, Roland Höglund, Mattias Miharada, Kenichi Nakamura, Yukio Hugues, Stephanie Greiff, Lennart Lindstedt, Malin Rosa, Fábio F. Pires, Cristiana F. Bassani-Sternberg, Michal Svane, Inge Marie Pereira, Carlos Filipe |
Issue Date: | 14-Jul-2023 | Publisher: | American Association for the Advancement of Science | Serial title, monograph or event: | Science Immunology | Volume: | 8 | Issue: | 85 | Abstract: | Decreased antigen presentation contributes to the ability of cancer cells to evade the immune system. We used the minimal gene regulatory network of type 1 conventional dendritic cells (cDC1) to reprogram cancer cells into professional antigen-presenting cells (tumor-APCs). Enforced expression of the transcription factors PU.1, IRF8, and BATF3 (PIB) was sufficient to induce the cDC1 phenotype in 36 cell lines derived from human and mouse hematological and solid tumors. Within 9 days of reprogramming, tumor-APCs acquired transcriptional and epigenetic programs associated with cDC1 cells. Reprogramming restored the expression of antigen presentation complexes and costimulatory molecules on the surfaces of tumor cells, allowing the presentation of endogenous tumor antigens on MHC-I and facilitating targeted killing by CD8+ T cells. Functionally, tumor-APCs engulfed and processed proteins and dead cells, secreted inflammatory cytokines, and cross-presented antigens to naïve CD8+ T cells. Human primary tumor cells could also be reprogrammed to increase their capability to present antigen and to activate patient-specific tumor-infiltrating lymphocytes. In addition to acquiring improved antigen presentation, tumor-APCs had impaired tumorigenicity in vitro and in vivo. Injection of in vitro generated melanoma-derived tumor-APCs into subcutaneous melanoma tumors delayed tumor growth and increased survival in mice. Antitumor immunity elicited by tumor-APCs was synergistic with immune checkpoint inhibitors. Our approach serves as a platform for the development of immunotherapies that endow cancer cells with the capability to process and present endogenous tumor antigens. | URI: | https://hdl.handle.net/10316/112596 | ISSN: | 2470-9468 | DOI: | 10.1126/sciimmunol.add4817 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Supplementary materials_Restoring-tumor-immunogenicity-with-dendritic-cell-reprogrammingScience-Immunology.pdf | 18.68 MB | Adobe PDF | View/Open | |
Restoring tumor immunogenicity with dendritic cell reprogramming.pdf | 4.42 MB | Adobe PDF | View/Open |
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