Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/112596
Title: Restoring tumor immunogenicity with dendritic cell reprogramming
Authors: Zimmermannova, Olga
Ferreira, Alexandra G. 
Ascic, Ervin
Velasco Santiago, Marta
Kurochkin, Ilia
Hansen, Morten
Met, Özcan
Caiado, Inês 
Shapiro, Ilja E.
Michaux, Justine
Humbert, Marion
Soto-Cabrera, Diego
Benonisson, Hreinn
Silvério-Alves, Rita 
Gomez-Jimenez, David
Bernardo, Carina
Bauden, Monika
Andersson, Roland
Höglund, Mattias
Miharada, Kenichi
Nakamura, Yukio
Hugues, Stephanie
Greiff, Lennart
Lindstedt, Malin
Rosa, Fábio F. 
Pires, Cristiana F.
Bassani-Sternberg, Michal
Svane, Inge Marie
Pereira, Carlos Filipe 
Issue Date: 14-Jul-2023
Publisher: American Association for the Advancement of Science
Serial title, monograph or event: Science Immunology
Volume: 8
Issue: 85
Abstract: Decreased antigen presentation contributes to the ability of cancer cells to evade the immune system. We used the minimal gene regulatory network of type 1 conventional dendritic cells (cDC1) to reprogram cancer cells into professional antigen-presenting cells (tumor-APCs). Enforced expression of the transcription factors PU.1, IRF8, and BATF3 (PIB) was sufficient to induce the cDC1 phenotype in 36 cell lines derived from human and mouse hematological and solid tumors. Within 9 days of reprogramming, tumor-APCs acquired transcriptional and epigenetic programs associated with cDC1 cells. Reprogramming restored the expression of antigen presentation complexes and costimulatory molecules on the surfaces of tumor cells, allowing the presentation of endogenous tumor antigens on MHC-I and facilitating targeted killing by CD8+ T cells. Functionally, tumor-APCs engulfed and processed proteins and dead cells, secreted inflammatory cytokines, and cross-presented antigens to naïve CD8+ T cells. Human primary tumor cells could also be reprogrammed to increase their capability to present antigen and to activate patient-specific tumor-infiltrating lymphocytes. In addition to acquiring improved antigen presentation, tumor-APCs had impaired tumorigenicity in vitro and in vivo. Injection of in vitro generated melanoma-derived tumor-APCs into subcutaneous melanoma tumors delayed tumor growth and increased survival in mice. Antitumor immunity elicited by tumor-APCs was synergistic with immune checkpoint inhibitors. Our approach serves as a platform for the development of immunotherapies that endow cancer cells with the capability to process and present endogenous tumor antigens.
URI: https://hdl.handle.net/10316/112596
ISSN: 2470-9468
DOI: 10.1126/sciimmunol.add4817
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

Show full item record

Page view(s)

41
checked on Jun 12, 2024

Download(s)

30
checked on Jun 12, 2024

Google ScholarTM

Check

Altmetric

Altmetric


This item is licensed under a Creative Commons License Creative Commons