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https://hdl.handle.net/10316/109768
Título: | Brain-derived neurotrophic factor promotes vasculature-associated migration of neuronal precursors toward the ischemic striatum | Autor: | Grade, Sofia Weng, Yuan C. Snapyan, Marina Kriz, Jasna Malva, João O. Saghatelyan, Armen |
Data: | 2013 | Editora: | Public Library of Science | Projeto: | This work was supported by an operating grant from the Canadian Institutes of Health Research (CIHR) to A.S. (MOP #110947), and student scholarships from Fundac¸a˜o Portuguesa para a Cieˆncia e a Tecnologia (FCT; SFRH/BD/32953/2006) and Fonds Que´be´cois de la Recherche sur la Nature et les Technologies (FQRNT; merit scholarship program for foreign students) to S.G. A.S. is the recipient of a Canada Research Chair in postnatal neurogenesis (950- 218183). | Título da revista, periódico, livro ou evento: | PLoS ONE | Volume: | 8 | Número: | 1 | Resumo: | Stroke induces the recruitment of neuronal precursors from the subventricular zone (SVZ) into the ischemic striatum. In injured areas, de-routed neuroblasts use blood vessels as a physical scaffold to their migration, in a process that resembles the constitutive migration seen in the rostral migratory stream (RMS). The molecular mechanism underlying injury-induced vasculature-mediated migration of neuroblasts in the post-stroke striatum remains, however, elusive. Using adult mice we now demonstrate that endothelial cells in the ischemic striatum produce brain-derived neurotrophic factor (BDNF), a neurotrophin that promotes the vasculature-mediated migration of neuronal precursors in the RMS, and that recruited neuroblasts maintain expression of p75NTR, a low-affinity receptor for BDNF. Reactive astrocytes, which are widespread throughout the damaged area, ensheath blood vessels and express TrkB, a high-affinity receptor for BDNF. Despite the absence of BDNF mRNA, we observed strong BDNF immunolabeling in astrocytes, suggesting that these glial cells trap extracellular BDNF. Importantly, this pattern of expression is reminiscent of the adult RMS, where TrkB-expressing astrocytes bind and sequester vasculature-derived BDNF, leading to the entry of migrating cells into the stationary phase. Real-time imaging of cell migration in acute brain slices revealed a direct role for BDNF in promoting the migration of neuroblasts to ischemic areas. We also demonstrated that cells migrating in the ischemic striatum display higher exploratory behavior and longer stationary periods than cells migrating in the RMS. Our findings suggest that the mechanisms involved in the injury-induced vasculature-mediated migration of neuroblasts recapitulate, at least partially, those observed during constitutive migration in the RMS. | URI: | https://hdl.handle.net/10316/109768 | ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0055039 | Direitos: | openAccess |
Aparece nas coleções: | FMUC Medicina - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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BrainDerived-Neurotrophic-Factor-Promotes-VasculatureAssociated-Migration-of-Neuronal-Precursors-toward-the-Ischemic-StriatumPLoS-ONE.pdf | 7.4 MB | Adobe PDF | Ver/Abrir |
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