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https://hdl.handle.net/10316/108920
Title: | An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes | Authors: | Medeiros, Maria C. R. Mestre, Ana Inácio, Pedro Asgarif, Sanaz Araújo, Inês M. Hubbard, Peter C. Velez, Zélia Cancela, M. Leonor Rocha, Paulo R. F. de Leeuw, Dago M. Biscarini, Fabio Gomes, Henrique L. |
Keywords: | Extracellular electrodes; Bioelectrical signals; Displacement current; Electrical double-layer; Intercellular signaling | Issue Date: | 2016 | Publisher: | Elsevier | Project: | “Implantable organic devices for advanced therapies (INNOVATE) PTDC/EEI-AUT/5442/2014) Intelligent Cell Surfaces (ICS), ref EuroBioSAS/0001/2010 UID/Multi/04326/2013 European Community Seventh Framework Programme (FP7/2007-2013) through the project iONE-FP7 grant agreement no. 280772 |
Serial title, monograph or event: | Sensing and Bio-Sensing Research | Volume: | 10 | Abstract: | An electrical method to measure extracellular bioelectrical activity in vitro is presented. This method exploits the Helmholtz capacitive double-layer established at the electrode surface. Small extracellular voltage variations in the order of μVs induce through the double-layer capacitor a displacement current that is measured. This current is then enhanced by a gain factor proportional to the electrode capacitance. In addition, when measurements are carried out at lowfrequencies in currentmode the electrode contribution to the noise can be minimized. The performance of the electrodes and themethod is demonstrated using zebrafish hearts and glioma cell cultures.We propose that this electricalmethod is an ideal tool to measure in vitro slow and temporally synchronized events that are often involved in long range intracellular signaling. | URI: | https://hdl.handle.net/10316/108920 | ISSN: | 22141804 | DOI: | 10.1016/j.sbsr.2016.06.002 | Rights: | openAccess |
Appears in Collections: | FCTUC Eng.Electrotécnica - Artigos em Revistas Internacionais I&D IT - Artigos em Revistas Internacionais |
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An electrical method to measure low-frequency collective and synchronized cell activity using extracellular electrodes.pdf | 1.48 MB | Adobe PDF | View/Open |
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