Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/108269
DC Field | Value | Language |
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dc.contributor.author | Baptista, Filipa I. | - |
dc.contributor.author | Aveleira, Célia A. | - |
dc.contributor.author | Castilho, Áurea F. | - |
dc.contributor.author | Ambrósio, António F. | - |
dc.date.accessioned | 2023-08-22T08:20:22Z | - |
dc.date.available | 2023-08-22T08:20:22Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0962-9351 | pt |
dc.identifier.issn | 1466-1861 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/108269 | - |
dc.description.abstract | Diabetic retinopathy is considered a neurovascular disorder, hyperglycemia being considered the main risk factor for this pathology. Diabetic retinopathy also presents features of a low-grade chronic inflammatory disease, including increased levels of cytokines in the retina, such as interleukin-1 beta (IL-1β). However, how high glucose and IL-1β affect the different retinal cell types remains to be clarified. In retinal neural cell cultures, we found that IL-1β and IL-1RI are present in microglia, macroglia, and neurons. Exposure of retinal neural cell cultures to high glucose upregulated both mRNA and protein levels of IL-1β. High glucose decreased microglial and macroglial cell proliferation, whereas IL-1β increased their proliferation. Interestingly, under high glucose condition, although the number of microglial cells decreased, they showed a less ramified morphology, suggesting a more activated state, as supported by the upregulation of the levels of ED-1, a marker of microglia activation. In conclusion, IL-1β might play a key role in diabetic retinopathy, affecting microglial and macroglial cells and ultimately contributing to neural changes observed in diabetic patients. Particularly, since IL-1β has an important role in retinal microglia activation and proliferation under diabetes, limiting IL-1β-triggered inflammatory processes may provide a new therapeutic strategy to prevent the progression of diabetic retinopathy. | pt |
dc.language.iso | eng | pt |
dc.publisher | Hindawi | pt |
dc.relation | This work was supported by the Foundation for Science and Technology (FCT), Portugal (SFRH/BPD/86830/2012; SFR H/BD/35961/2007; SFRH/BD/30235/2006; SFRH/BPD/111 710/2015; SFRH/BPD/73942/2010; SFRH/BD/18827/2004), Strategic Project PEst-C/SAU/UI3282/2011-2013 and UID/ NEU/04539/2013 (FCT, Portugal, and COMPETE-FEDER), COMPETE-FEDER (POCI-01-0145-FEDER-007440), and Centro 2020 Regional Operational Programme (CENTRO- 01-0145-FEDER-000008: BrainHealth 2020). | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Cell Proliferation | pt |
dc.subject.mesh | Cell Survival | pt |
dc.subject.mesh | Cells, Cultured | pt |
dc.subject.mesh | Diabetes Mellitus, Experimental | pt |
dc.subject.mesh | Enzyme-Linked Immunosorbent Assay | pt |
dc.subject.mesh | Glucose | pt |
dc.subject.mesh | Immunohistochemistry | pt |
dc.subject.mesh | In Situ Nick-End Labeling | pt |
dc.subject.mesh | Interleukin-1beta | pt |
dc.subject.mesh | Microglia | pt |
dc.subject.mesh | Neuroglia | pt |
dc.subject.mesh | Rats | pt |
dc.subject.mesh | Rats, Wistar | pt |
dc.subject.mesh | Real-Time Polymerase Chain Reaction | pt |
dc.title | Elevated Glucose and Interleukin-1β Differentially Affect Retinal Microglial Cell Proliferation | pt |
dc.type | article | - |
degois.publication.firstPage | 4316316 | pt |
degois.publication.lastPage | 11 | pt |
degois.publication.title | Mediators of Inflammation | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1155/2017/4316316 | pt |
degois.publication.volume | 2017 | pt |
dc.date.embargo | 2017-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-7972-640X | - |
crisitem.author.orcid | 0000-0002-0477-1641 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais I&D IBILI - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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Elevated-Glucose-and-Interleukin1--Differentially-Affect-Retinal-Microglial-Cell-ProliferationMediators-of-Inflammation.pdf | 5.21 MB | Adobe PDF | View/Open |
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