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Title: Elevated Glucose and Interleukin-1β Differentially Affect Retinal Microglial Cell Proliferation
Authors: Baptista, Filipa I. 
Aveleira, Célia A. 
Castilho, Áurea F. 
Ambrósio, António F. 
Issue Date: 2017
Publisher: Hindawi
Project: This work was supported by the Foundation for Science and Technology (FCT), Portugal (SFRH/BPD/86830/2012; SFR H/BD/35961/2007; SFRH/BD/30235/2006; SFRH/BPD/111 710/2015; SFRH/BPD/73942/2010; SFRH/BD/18827/2004), Strategic Project PEst-C/SAU/UI3282/2011-2013 and UID/ NEU/04539/2013 (FCT, Portugal, and COMPETE-FEDER), COMPETE-FEDER (POCI-01-0145-FEDER-007440), and Centro 2020 Regional Operational Programme (CENTRO- 01-0145-FEDER-000008: BrainHealth 2020). 
Serial title, monograph or event: Mediators of Inflammation
Volume: 2017
Abstract: Diabetic retinopathy is considered a neurovascular disorder, hyperglycemia being considered the main risk factor for this pathology. Diabetic retinopathy also presents features of a low-grade chronic inflammatory disease, including increased levels of cytokines in the retina, such as interleukin-1 beta (IL-1β). However, how high glucose and IL-1β affect the different retinal cell types remains to be clarified. In retinal neural cell cultures, we found that IL-1β and IL-1RI are present in microglia, macroglia, and neurons. Exposure of retinal neural cell cultures to high glucose upregulated both mRNA and protein levels of IL-1β. High glucose decreased microglial and macroglial cell proliferation, whereas IL-1β increased their proliferation. Interestingly, under high glucose condition, although the number of microglial cells decreased, they showed a less ramified morphology, suggesting a more activated state, as supported by the upregulation of the levels of ED-1, a marker of microglia activation. In conclusion, IL-1β might play a key role in diabetic retinopathy, affecting microglial and macroglial cells and ultimately contributing to neural changes observed in diabetic patients. Particularly, since IL-1β has an important role in retinal microglia activation and proliferation under diabetes, limiting IL-1β-triggered inflammatory processes may provide a new therapeutic strategy to prevent the progression of diabetic retinopathy.
ISSN: 0962-9351
DOI: 10.1155/2017/4316316
Rights: openAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
I&D IBILI - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais

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