Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/108268
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dc.contributor.authorMachado-Pereira, Marta-
dc.contributor.authorSantos, Tiago-
dc.contributor.authorFerreira, Lino-
dc.contributor.authorBernardino, Liliana-
dc.contributor.authorFerreira, Raquel-
dc.date.accessioned2023-08-22T08:15:13Z-
dc.date.available2023-08-22T08:15:13Z-
dc.date.issued2017-
dc.identifier.issn0962-9351pt
dc.identifier.issn1466-1861pt
dc.identifier.urihttps://hdl.handle.net/10316/108268-
dc.description.abstractInflammatory mechanisms triggered by microglial cells are involved in the pathophysiology of several brain disorders, hindering repair. Herein, we propose the use of retinoic acid-loaded polymeric nanoparticles (RA-NP) as a means to modulate microglia response towards an anti-inflammatory and neuroprotective phenotype (M2). RA-NP were first confirmed to be internalized by N9 microglial cells; nanoparticles did not affect cell survival at concentrations below 100 μg/mL. Then, immunocytochemical studies were performed to assess the expression of pro- and anti-inflammatory mediators. Our results show that RA-NP inhibited LPS-induced release of nitric oxide and the expression of inducible nitric oxide synthase and promoted arginase-1 and interleukin-4 production. Additionally, RA-NP induced a ramified microglia morphology (indicative of M2 state), promoting tissue viability, particularly neuronal survival, and restored the expression of postsynaptic protein-95 in organotypic hippocampal slice cultures exposed to an inflammatory challenge. RA-NP also proved to be more efficient than the free equivalent RA concentration. Altogether, our data indicate that RA-NP may be envisioned as a promising therapeutic agent for brain inflammatory diseases.pt
dc.language.isoengpt
dc.publisherHindawipt
dc.relationPOCI- 01-0145-FEDER-007491pt
dc.relationFCOMP-01-0124-FEDER- 041099pt
dc.relationUID/Multi/00709/2013pt
dc.relationEXPL/ BIM-MED/0822/2013pt
dc.relationSFRH/BPD/ 94228/2013pt
dc.relationSFRH/BD/79526/2011pt
dc.relationL’Oréal-UNESCO Portugal for Women in Sciencept
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subject.meshAnimalspt
dc.subject.meshAnti-Inflammatory Agentspt
dc.subject.meshCell Linept
dc.subject.meshCell Survivalpt
dc.subject.meshHippocampuspt
dc.subject.meshImmunohistochemistrypt
dc.subject.meshLipopolysaccharidespt
dc.subject.meshMicept
dc.subject.meshMice, Inbred C57BLpt
dc.subject.meshMicrogliapt
dc.subject.meshNanoparticlespt
dc.subject.meshTretinoinpt
dc.titleAnti-Inflammatory Strategy for M2 Microglial Polarization Using Retinoic Acid-Loaded Nanoparticlespt
dc.typearticle-
degois.publication.firstPage6742427pt
degois.publication.lastPage11pt
degois.publication.titleMediators of Inflammationpt
dc.peerreviewedyespt
dc.identifier.doi10.1155/2017/6742427pt
degois.publication.volume2017pt
dc.date.embargo2017-01-01*
uc.date.periodoEmbargo0pt
item.fulltextCom Texto completo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.openairetypearticle-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcidhttps://orcid.org/0000-0003-0237-499X-
crisitem.author.orcid0000-0001-8985-9302-
Appears in Collections:IIIUC - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
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