Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/108268
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Machado-Pereira, Marta | - |
dc.contributor.author | Santos, Tiago | - |
dc.contributor.author | Ferreira, Lino | - |
dc.contributor.author | Bernardino, Liliana | - |
dc.contributor.author | Ferreira, Raquel | - |
dc.date.accessioned | 2023-08-22T08:15:13Z | - |
dc.date.available | 2023-08-22T08:15:13Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 0962-9351 | pt |
dc.identifier.issn | 1466-1861 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/108268 | - |
dc.description.abstract | Inflammatory mechanisms triggered by microglial cells are involved in the pathophysiology of several brain disorders, hindering repair. Herein, we propose the use of retinoic acid-loaded polymeric nanoparticles (RA-NP) as a means to modulate microglia response towards an anti-inflammatory and neuroprotective phenotype (M2). RA-NP were first confirmed to be internalized by N9 microglial cells; nanoparticles did not affect cell survival at concentrations below 100 μg/mL. Then, immunocytochemical studies were performed to assess the expression of pro- and anti-inflammatory mediators. Our results show that RA-NP inhibited LPS-induced release of nitric oxide and the expression of inducible nitric oxide synthase and promoted arginase-1 and interleukin-4 production. Additionally, RA-NP induced a ramified microglia morphology (indicative of M2 state), promoting tissue viability, particularly neuronal survival, and restored the expression of postsynaptic protein-95 in organotypic hippocampal slice cultures exposed to an inflammatory challenge. RA-NP also proved to be more efficient than the free equivalent RA concentration. Altogether, our data indicate that RA-NP may be envisioned as a promising therapeutic agent for brain inflammatory diseases. | pt |
dc.language.iso | eng | pt |
dc.publisher | Hindawi | pt |
dc.relation | POCI- 01-0145-FEDER-007491 | pt |
dc.relation | FCOMP-01-0124-FEDER- 041099 | pt |
dc.relation | UID/Multi/00709/2013 | pt |
dc.relation | EXPL/ BIM-MED/0822/2013 | pt |
dc.relation | SFRH/BPD/ 94228/2013 | pt |
dc.relation | SFRH/BD/79526/2011 | pt |
dc.relation | L’Oréal-UNESCO Portugal for Women in Science | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Anti-Inflammatory Agents | pt |
dc.subject.mesh | Cell Line | pt |
dc.subject.mesh | Cell Survival | pt |
dc.subject.mesh | Hippocampus | pt |
dc.subject.mesh | Immunohistochemistry | pt |
dc.subject.mesh | Lipopolysaccharides | pt |
dc.subject.mesh | Mice | pt |
dc.subject.mesh | Mice, Inbred C57BL | pt |
dc.subject.mesh | Microglia | pt |
dc.subject.mesh | Nanoparticles | pt |
dc.subject.mesh | Tretinoin | pt |
dc.title | Anti-Inflammatory Strategy for M2 Microglial Polarization Using Retinoic Acid-Loaded Nanoparticles | pt |
dc.type | article | - |
degois.publication.firstPage | 6742427 | pt |
degois.publication.lastPage | 11 | pt |
degois.publication.title | Mediators of Inflammation | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1155/2017/6742427 | pt |
degois.publication.volume | 2017 | pt |
dc.date.embargo | 2017-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.cerifentitytype | Publications | - |
crisitem.project.grantno | Health Sciences Research Centre | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | https://orcid.org/0000-0003-0237-499X | - |
crisitem.author.orcid | 0000-0001-8985-9302 | - |
Appears in Collections: | IIIUC - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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AntiInflammatory-Strategy-for-M2-Microglial-Polarization-Using-Retinoic-AcidLoaded-NanoparticlesMediators-of-Inflammation.pdf | 3.36 MB | Adobe PDF | View/Open |
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