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https://hdl.handle.net/10316/108268
Title: | Anti-Inflammatory Strategy for M2 Microglial Polarization Using Retinoic Acid-Loaded Nanoparticles | Authors: | Machado-Pereira, Marta Santos, Tiago Ferreira, Lino Bernardino, Liliana Ferreira, Raquel |
Issue Date: | 2017 | Publisher: | Hindawi | Project: | FCOMP-01-0124-FEDER- 041099 UID/Multi/00709/2013 EXPL/ BIM-MED/0822/2013 SFRH/BPD/ 94228/2013 SFRH/BD/79526/2011 |
Serial title, monograph or event: | Mediators of Inflammation | Volume: | 2017 | Abstract: | Inflammatory mechanisms triggered by microglial cells are involved in the pathophysiology of several brain disorders, hindering repair. Herein, we propose the use of retinoic acid-loaded polymeric nanoparticles (RA-NP) as a means to modulate microglia response towards an anti-inflammatory and neuroprotective phenotype (M2). RA-NP were first confirmed to be internalized by N9 microglial cells; nanoparticles did not affect cell survival at concentrations below 100 μg/mL. Then, immunocytochemical studies were performed to assess the expression of pro- and anti-inflammatory mediators. Our results show that RA-NP inhibited LPS-induced release of nitric oxide and the expression of inducible nitric oxide synthase and promoted arginase-1 and interleukin-4 production. Additionally, RA-NP induced a ramified microglia morphology (indicative of M2 state), promoting tissue viability, particularly neuronal survival, and restored the expression of postsynaptic protein-95 in organotypic hippocampal slice cultures exposed to an inflammatory challenge. RA-NP also proved to be more efficient than the free equivalent RA concentration. Altogether, our data indicate that RA-NP may be envisioned as a promising therapeutic agent for brain inflammatory diseases. | URI: | https://hdl.handle.net/10316/108268 | ISSN: | 0962-9351 1466-1861 |
DOI: | 10.1155/2017/6742427 | Rights: | openAccess |
Appears in Collections: | IIIUC - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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