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https://hdl.handle.net/10316/107991
Título: | Errors in protein synthesis increase the level of saturated fatty acids and affect the overall lipid profiles of yeast | Autor: | Araújo, Ana Rita D. Melo, Tânia Maciel, Elisabete A. Pereira, Clara Morais, Catarina M. Santinha, Deolinda R. Tavares, Joana F. Oliveira, Helena Jurado, Amália Costa, Vítor Domingues, Pedro Domingues, Maria Rosário M. Santos, Manuel A. S. |
Data: | 2018 | Editora: | Public Library of Science | Projeto: | FCT-ANR/IMI-MIC/0041/2012 PTDC/ IMI-MIC/5350/2014 UID/BIM/04501/2013 PTDC/QUI-BIQ/104968/ 2008 SFRH/BD/78835/2011 SFRH/BD/79077/2011 SFRH/BPD/104165/2014 SFRH/BPD/111736/2015 BPD/UI51/5388/ 2017 IF/00889/2015 |
Título da revista, periódico, livro ou evento: | PLoS ONE | Volume: | 13 | Número: | 8 | Resumo: | The occurrence of protein synthesis errors (mistranslation) above the typical mean mistranslation level of 10-4 is mostly deleterious to yeast, zebrafish and mammal cells. Previous yeast studies have shown that mistranslation affects fitness and deregulates genes related to lipid metabolism, but there is no experimental proof that such errors alter yeast lipid profiles. We engineered yeast strains to misincorporate serine at alanine and glycine sites on a global scale and evaluated the putative effects on the lipidome. Lipids from whole cells were extracted and analysed by thin layer chromatography (TLC), liquid chromatography-mass spectrometry(LC-MS) and gas chromatography (GC). Oxidative damage, fatty acid desaturation and membrane fluidity changes were screened to identify putative alterations in lipid profiles in both logarithmic (fermentative) and post-diauxic shift (respiratory) phases. There were alterations in several lipid classes, namely lyso-phosphatidylcholine, phosphatidic acid, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and triglyceride, and in the fatty acid profiles, namely C16:1, C16:0, C18:1 and C18:0. Overall, the relative content of lipid species with saturated FA increased in detriment of those with unsaturated fatty acids. The expression of the OLE1 mRNA was deregulated, but phospholipid fluidity changes were not observed. These data expand current knowledge of mistranslation biology and highlight its putative roles in human diseases. | URI: | https://hdl.handle.net/10316/107991 | ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0202402 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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Ficheiro | Descrição | Tamanho | Formato | |
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Errors-in-protein-synthesis-increase-the-level-of-saturated-fatty-acids-and-affect-the-overall-lipid-profiles-of-yeastPLoS-ONE.pdf | 6.3 MB | Adobe PDF | Ver/Abrir |
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