Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/107624
Title: BDNF-Live-Exon-Visualization (BLEV) Allows Differential Detection of BDNF Transcripts in vitro and in vivo
Authors: Singer, Wibke
Manthey, Marie
Panford-Walsh, Rama
Matt, Lucas
Geisler, Hyun-Soon
Passeri, Eleonora
Baj, Gabriele
Tongiorgi, Enrico 
Leal, Graciano 
Duarte, Carlos B. 
Salazar, Ivan L. 
Eckert, Philipp
Rohbock, Karin
Hu, Jing
Strotmann, Jörg
Ruth, Peter
Zimmermann, Ulrike
Rüttiger, Lukas
Ott, Thomas
Schimmang, Thomas
Knipper, Marlies
Keywords: BDNF detection; Bdnf exon-IV and -VI; activity-dependent BDNF expression; long-lasting plasticity changes; transgenic BDNF reporter mice
Issue Date: 2018
Publisher: Frontiers Media S.A.
Project: PEst-C/SAU/LA0001/2013 
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2013/PT 
UID/BIM/4501/2013 
PTDC/MED-NEU/28656/2017 
CENTRO-01-0145-FEDER-000008/BrainHealth2020 
Serial title, monograph or event: Frontiers in Molecular Neuroscience
Volume: 11
Abstract: Bdnf exon-IV and exon-VI transcripts are driven by neuronal activity and are involved in pathologies related to sleep, fear or memory disorders. However, how their differential transcription translates activity changes into long-lasting network changes is elusive. Aiming to trace specifically the network controlled by exon-IV and -VI derived BDNF during activity-dependent plasticity changes, we generated a transgenic reporter mouse for B DNF- l ive- e xon- v isualization (BLEV), in which expression of Bdnf exon-IV and -VI can be visualized by co-expression of CFP and YFP. CFP and YFP expression was differentially activated and targeted in cell lines, primary cultures and BLEV reporter mice without interfering with BDNF protein synthesis. CFP and YFP expression, moreover, overlapped with BDNF protein expression in defined hippocampal neuronal, glial and vascular locations in vivo. So far, activity-dependent BDNF cannot be explicitly monitored independent of basal BDNF levels. The BLEV reporter mouse therefore provides a new model, which can be used to test whether stimulus-induced activity-dependent changes in BDNF expression are instrumental for long-lasting plasticity modifications.
URI: https://hdl.handle.net/10316/107624
ISSN: 1662-5099
DOI: 10.3389/fnmol.2018.00325
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

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