Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/106580
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Almeida, Mariana C. | - |
dc.contributor.author | Branco, Rita | - |
dc.contributor.author | Morais, Paula V. | - |
dc.date.accessioned | 2023-04-12T07:53:13Z | - |
dc.date.available | 2023-04-12T07:53:13Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1932-6203 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/106580 | - |
dc.description.abstract | Vanadium is a transition metal that has been added recently to the EU list of Raw Critical Metals. The growing needs of vanadium primarily in the steel industry justify its increasing economic value. However, because mining of vanadium sources (i. e. ores, concentrates and vanadiferous slags) is expanding, so is vanadium environmental contamination. Bioleaching comes forth as smart strategy to deal with supply demand and environmental contamination. It requires organisms that are able to mobilize the metal and at the same time are resistant to the leachate generated. Here, we investigated the molecular mechanisms underlying vanadium resistance in Ochrobactrum tritici strains. The highly resistant strain 5bvl1 was able to grow at concentrations > 30 mM vanadate, while the O. tritici type strain only tolerated < 3 mM vanadate concentrations. Screening of O. tritici single mutants (chrA, chrC, chrF and recA) growth during vanadate exposure revealed that vanadate resistance was associated with chromate resistance mechanisms (in particular ChrA, an efflux pump and ChrC, a superoxide dismutase). We also showed that sensitivity to vanadate was correlated with increased accumulation of vanadate intracellularly, while in resistant cells this was not found. Other up-regulated proteins found during vanadate exposure were ABC transporters for methionine and iron, suggesting that cellular responses to vanadate toxicity may also induce changes in unspecific transport and chelation of vanadate. | pt |
dc.language.iso | eng | pt |
dc.publisher | Public Library of Science | pt |
dc.relation | UID/ EMS/00285/2013 | pt |
dc.relation | PTW-PTDC/AAG-REC/3839/ 2014 | pt |
dc.relation | ERA-MIN/0002/2015 | pt |
dc.relation | POCI-01-0145- FEDER-031820 | pt |
dc.relation | Renature - Centro-01-0145- FEDER-000007 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Arsenic | pt |
dc.subject.mesh | Bacterial Proteins | pt |
dc.subject.mesh | Chromates | pt |
dc.subject.mesh | Chromium | pt |
dc.subject.mesh | Drug Resistance, Bacterial | pt |
dc.subject.mesh | Microbial Sensitivity Tests | pt |
dc.subject.mesh | Ochrobactrum | pt |
dc.subject.mesh | Proteome | pt |
dc.subject.mesh | Vanadates | pt |
dc.subject.mesh | Vanadium | pt |
dc.title | Response to vanadate exposure in Ochrobactrum tritici strains | pt |
dc.type | article | - |
degois.publication.firstPage | e0229359 | pt |
degois.publication.issue | 2 | pt |
degois.publication.title | PLoS ONE | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1371/journal.pone.0229359 | pt |
degois.publication.volume | 15 | pt |
dc.date.embargo | 2020-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.researchunit | MARE - Marine and Environmental Sciences Centre | - |
crisitem.author.orcid | 0000-0003-0291-8029 | - |
crisitem.author.orcid | 0000-0002-1939-6389 | - |
Aparece nas coleções: | I&D CEMMPRE - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Response-to-vanadate-exposure-in-Ochrobactrum-tritici-strainsPLoS-ONE.pdf | 1.9 MB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
6
Visto em 30/set/2024
Citações WEB OF SCIENCETM
6
Visto em 2/out/2024
Visualizações de página
76
Visto em 1/out/2024
Downloads
25
Visto em 1/out/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Este registo está protegido por Licença Creative Commons