Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/106580
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dc.contributor.authorAlmeida, Mariana C.-
dc.contributor.authorBranco, Rita-
dc.contributor.authorMorais, Paula V.-
dc.date.accessioned2023-04-12T07:53:13Z-
dc.date.available2023-04-12T07:53:13Z-
dc.date.issued2020-
dc.identifier.issn1932-6203pt
dc.identifier.urihttps://hdl.handle.net/10316/106580-
dc.description.abstractVanadium is a transition metal that has been added recently to the EU list of Raw Critical Metals. The growing needs of vanadium primarily in the steel industry justify its increasing economic value. However, because mining of vanadium sources (i. e. ores, concentrates and vanadiferous slags) is expanding, so is vanadium environmental contamination. Bioleaching comes forth as smart strategy to deal with supply demand and environmental contamination. It requires organisms that are able to mobilize the metal and at the same time are resistant to the leachate generated. Here, we investigated the molecular mechanisms underlying vanadium resistance in Ochrobactrum tritici strains. The highly resistant strain 5bvl1 was able to grow at concentrations > 30 mM vanadate, while the O. tritici type strain only tolerated < 3 mM vanadate concentrations. Screening of O. tritici single mutants (chrA, chrC, chrF and recA) growth during vanadate exposure revealed that vanadate resistance was associated with chromate resistance mechanisms (in particular ChrA, an efflux pump and ChrC, a superoxide dismutase). We also showed that sensitivity to vanadate was correlated with increased accumulation of vanadate intracellularly, while in resistant cells this was not found. Other up-regulated proteins found during vanadate exposure were ABC transporters for methionine and iron, suggesting that cellular responses to vanadate toxicity may also induce changes in unspecific transport and chelation of vanadate.pt
dc.language.isoengpt
dc.publisherPublic Library of Sciencept
dc.relationUID/ EMS/00285/2013pt
dc.relationPTW-PTDC/AAG-REC/3839/ 2014pt
dc.relationERA-MIN/0002/2015pt
dc.relationPOCI-01-0145- FEDER-031820pt
dc.relationRenature - Centro-01-0145- FEDER-000007pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subject.meshArsenicpt
dc.subject.meshBacterial Proteinspt
dc.subject.meshChromatespt
dc.subject.meshChromiumpt
dc.subject.meshDrug Resistance, Bacterialpt
dc.subject.meshMicrobial Sensitivity Testspt
dc.subject.meshOchrobactrumpt
dc.subject.meshProteomept
dc.subject.meshVanadatespt
dc.subject.meshVanadiumpt
dc.titleResponse to vanadate exposure in Ochrobactrum tritici strainspt
dc.typearticle-
degois.publication.firstPagee0229359pt
degois.publication.issue2pt
degois.publication.titlePLoS ONEpt
dc.peerreviewedyespt
dc.identifier.doi10.1371/journal.pone.0229359pt
degois.publication.volume15pt
dc.date.embargo2020-01-01*
uc.date.periodoEmbargo0pt
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairetypearticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
crisitem.author.researchunitCEMMPRE - Centre for Mechanical Engineering, Materials and Processes-
crisitem.author.researchunitMARE - Marine and Environmental Sciences Centre-
crisitem.author.orcid0000-0003-0291-8029-
crisitem.author.orcid0000-0002-1939-6389-
Appears in Collections:I&D CEMMPRE - Artigos em Revistas Internacionais
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