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Title: Blueberry Consumption Challenges Hepatic Mitochondrial Bioenergetics and Elicits Transcriptomics Reprogramming in Healthy Wistar Rats
Authors: Nunes, Sara 
Viana, Sofia D. 
Preguiça, Inês 
Alves, André 
Fernandes, Rosa 
Teodoro, João S. 
Figueirinha, Artur 
Salgueiro, Lígia 
Silva, Sara
Jarak, Ivana 
Carvalho, Rui A. 
Cavadas, Cláudia 
Rolo, Anabela P. 
Palmeira, Carlos M. 
Pintado, Maria M.
Reis, Flávio 
Keywords: blueberries; long-term consumption; bioenergetics remodeling; transcriptomics reprogramming; anti-inflammatory pre-conditioning
Issue Date: 14-Nov-2020
Publisher: MDPI
Project: This research was funded by the European Regional Development Fund (FEDER), through Programa Operacional Factores de Competitividade COMPETE2020 (CENTRO-01-0145-FEDER-000012-HealthyAging2020) and by National funds via Portuguese Science and Technology Foundation (FCT): Strategic Projects UID/NEU/04539/2013, UID/NEU/04539/2019, UIDB/04539/2020 and UIDP/04539/2020 (CIBB), SFRH/BD/109017/2015 (PhD Fellowship) and PTDC/SAU-NUT/31712/2017, as well as by COMPETE-FEDER funds (POCI-01-0145-FEDER-007440 and POCI-01-0145-FEDER-031712). BB juice phytochemical analyses received financial support from PT national funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through Grant UIDB/50006/2020 and by Programa de Cooperación Interreg V-A España—Portugal (POCTEP) 2014–2020 (project 0377_IBERPHENOL_6_E). J.S.T. is a CNC assistant researcher, contracted under the CEEC program (CEECIND/04400/2017). NMR data was collected at the UC-NMR facility, which is supported in part by the FEDER—European Regional Development Fund through the COMPETE Programme (Operational Programme for Competitiveness) and by National Funds through FCT—Fundação para a Ciência e a Tecnologia (Portuguese Foundation for Science and Technology) through grants REEQ/481/QUI/2006, RECI/QEQ-QFI/0168/2012, CENTRO-07-CT62-FEDER-002012, and Rede Nacional de Ressonância Magnética Nuclear (RNRMN) 
Serial title, monograph or event: Pharmaceutics
Volume: 12
Issue: 11
Abstract: An emergent trend of blueberries' (BB) "prophylactic" consumption, due to their phytochemicals' richness and well-known health-promoting claims, is widely scaled-up. However, the benefits arising from BB indiscriminate intake remains puzzling based on incongruent preclinical and human data. To provide a more in-depth elucidation and support towards a healthier and safer consumption, we conducted a translation-minded experimental study in healthy Wistar rats that consumed BB in a juice form (25 g/kg body weight (BW)/day; 14 weeks' protocol). Particular attention was paid to the physiological adaptations succeeding in the gut and liver tissues regarding the acknowledged BB-induced metabolic benefits. Systemically, BB boosted serum antioxidant activity and repressed the circulating levels of 3-hydroxybutyrate (3-HB) ketone bodies and 3-HB/acetoacetate ratio. Moreover, BB elicited increased fecal succinic acid levels without major changes on gut microbiota (GM) composition and gut ultra-structural organization. Remarkably, an accentuated hepatic mitochondrial bioenergetic challenge, ensuing metabolic transcriptomic reprogramming along with a concerted anti-inflammatory pre-conditioning, was clearly detected upon long-term consumption of BB phytochemicals. Altogether, the results disclosed herein portray a quiescent mitochondrial-related metabolomics and hint for a unified adaptive response to this nutritional challenge. The beneficial or noxious consequences arising from this dietary trend should be carefully interpreted and necessarily claims future research.
ISSN: 1999-4923
DOI: 10.3390/pharmaceutics12111094
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D CIBB - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
I&D CIEPQPF - Artigos em Revistas Internacionais

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