Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/106208
DC FieldValueLanguage
dc.contributor.authorCosta, Eduardo-
dc.contributor.authorFerreira-Gonçalves, Tânia-
dc.contributor.authorCardoso, Miguel-
dc.contributor.authorCoelho, João M. P.-
dc.contributor.authorGaspar, Maria Manuela-
dc.contributor.authorFaísca, Pedro-
dc.contributor.authorAscensão, Lia-
dc.contributor.authorCabrita, António S.-
dc.contributor.authorReis, Catarina Pinto-
dc.contributor.authorFigueiredo, Isabel V.-
dc.date.accessioned2023-03-24T12:32:50Z-
dc.date.available2023-03-24T12:32:50Z-
dc.date.issued2020-12-18-
dc.identifier.issn1422-0067pt
dc.identifier.urihttps://hdl.handle.net/10316/106208-
dc.description.abstractBreast cancer is one of the most frequently diagnosed malignancies and common causes of cancer death in women. Recent studies suggest that environmental exposures to certain chemicals, such as 7,12-Dimethylbenzanthracene (DMBA), a chemical present in tobacco, may increase the risk of developing breast cancer later in life. The first-line treatments for breast cancer (surgery, chemotherapy or a combination of both) are generally invasive and frequently associated with severe side effects and high comorbidity. Consequently, novel approaches are strongly required to find more natural-like experimental models that better reflect the tumors' etiology, physiopathology and response to treatments, as well as to find more targeted, efficient and minimally invasive treatments. This study proposes the development and an in deep biological characterization of an experimental model using DMBA-tumor-induction in Sprague-Dawley female rats. Moreover, a photothermal therapy approach using a near-infrared laser coupled with gold nanoparticles was preliminarily assessed. The gold nanoparticles were functionalized with Epidermal Growth Factor, and their physicochemical properties and in vitro effects were characterized. DMBA proved to be a very good and selective inductor of breast cancer, with 100% incidence and inducing an average of 4.7 tumors per animal. Epigenetic analysis showed that tumors classified with worst prognosis were hypomethylated. The tumor-induced rats were then subjected to a preliminary treatment using functionalized gold nanoparticles and its activation by laser (650-900 nm). The treatment outcomes presented very promising alterations in terms of tumor histology, confirming the presence of necrosis in most of the cases. Although this study revealed encouraging results as a breast cancer therapy, it is important to define tumor eligibility and specific efficiency criteria to further assess its application in breast cancer treatment on other species.pt
dc.language.isoengpt
dc.publisherMDPIpt
dc.relationUIDB/00645/2020pt
dc.relationUID/DTP/04138/2019pt
dc.relationSFRH/BD/147306/2019pt
dc.relationUIDP/50017/2020pt
dc.relationUIDB/50017/2020pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subjectbreast cancerpt
dc.subjectexperimental modelpt
dc.subjectDMBApt
dc.subjectlaser photothermal therapypt
dc.subjectgold nanoparticlespt
dc.subjectepigenetic alterationspt
dc.subject.mesh5-Methylcytosinept
dc.subject.mesh9,10-Dimethyl-1,2-benzanthracenept
dc.subject.meshAnimalspt
dc.subject.meshBody Weightpt
dc.subject.meshFemalept
dc.subject.meshGoldpt
dc.subject.meshMammary Neoplasms, Experimentalpt
dc.subject.meshMetal Nanoparticlespt
dc.subject.meshRatspt
dc.subject.meshRats, Sprague-Dawleypt
dc.subject.meshHyperthermia, Inducedpt
dc.subject.meshModels, Theoreticalpt
dc.titleA Step Forward in Breast Cancer Research: From a Natural-Like Experimental Model to a Preliminary Photothermal Approachpt
dc.typearticle-
degois.publication.firstPage9681pt
degois.publication.issue24pt
degois.publication.titleInternational Journal of Molecular Sciencespt
dc.peerreviewedyespt
dc.identifier.doi10.3390/ijms21249681pt
degois.publication.volume21pt
dc.date.embargo2020-12-18*
uc.date.periodoEmbargo0pt
item.grantfulltextopen-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairetypearticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextCom Texto completo-
crisitem.author.deptFaculty of Medicine-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.orcid0000-0002-2295-5532-
crisitem.author.orcid0000-0001-9170-4334-
Appears in Collections:FFUC- Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
FMUC Med. Dentária - Artigos em Revistas Internacionais
I&D ICBR - Artigos em Revistas Internacionais
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