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|Title:||(+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line||Authors:||Souto, Eliana B.
Souto, Selma B
Atanasov, Atanas G.
Andrade, Luciana N.
|Keywords:||(+)-limonene 1; 2-epoxide; monoterpene; Imwitor 900K-SLN; lipid peroxidation; cytotoxicity; HaCaT cell line||Issue Date:||20-Feb-2020||Publisher:||MDPI||Project:||CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico
CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)
FAPITEC/SE (Fundação de Apoio à Pesquisa e Inovação Tecnológica do Estado de Sergipe)
|Serial title, monograph or event:||International Journal of Molecular Sciences||Volume:||21||Issue:||4||Abstract:||In this work, we developed a solid lipid nanoparticle (SLN) formulation with (+)-limonene 1,2-epoxide and glycerol monostearate (Lim-SLNs), stabilized with Poloxamer® 188 in aqueous dispersion to modify the release profile of the loaded monoterpene derivative. We also evaluated the role of SLNs in lipid peroxidation and cytotoxicity in a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin (the HaCaT cell line). For the cell viability assay, the colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used. Lim-SLNs with a loading capacity and encapsulation efficiency of 0.39% and 63%, respectively, were produced by high pressure homogenization. A mean particle size of 194 ± 3.4 nm and polydispersity index of 0.244 were recorded for the loaded Lim-SLNs, as compared to 203 ± 1.5 nm (PI 0.213) for the non-loaded (blank) SLNs. The loading of the monoterpene derivative into glycerol monostearate SLNs fitted into the zero-order kinetics, and ameliorated both lipid peroxidation and cytotoxicity in a keratinocyte cell line. A promising formulation for antioxidant and anti-tumoral activities is here proposed.||URI:||http://hdl.handle.net/10316/106193||ISSN:||1422-0067||DOI:||10.3390/ijms21041449||Rights:||openAccess|
|Appears in Collections:||FFUC- Artigos em Revistas Internacionais|
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