Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/106193
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Souto, Eliana B. | - |
dc.contributor.author | Zielińska, Aleksandra | - |
dc.contributor.author | Souto, Selma B | - |
dc.contributor.author | Durazzo, Alessandra | - |
dc.contributor.author | Lucarini, Massimo | - |
dc.contributor.author | Santini, Antonello | - |
dc.contributor.author | Atanasov, Atanas G. | - |
dc.contributor.author | Marques, Conrado | - |
dc.contributor.author | Andrade, Luciana N. | - |
dc.contributor.author | Severino, Patrícia | - |
dc.date.accessioned | 2023-03-24T09:48:17Z | - |
dc.date.available | 2023-03-24T09:48:17Z | - |
dc.date.issued | 2020-02-20 | - |
dc.identifier.issn | 1422-0067 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/106193 | - |
dc.description.abstract | In this work, we developed a solid lipid nanoparticle (SLN) formulation with (+)-limonene 1,2-epoxide and glycerol monostearate (Lim-SLNs), stabilized with Poloxamer® 188 in aqueous dispersion to modify the release profile of the loaded monoterpene derivative. We also evaluated the role of SLNs in lipid peroxidation and cytotoxicity in a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin (the HaCaT cell line). For the cell viability assay, the colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used. Lim-SLNs with a loading capacity and encapsulation efficiency of 0.39% and 63%, respectively, were produced by high pressure homogenization. A mean particle size of 194 ± 3.4 nm and polydispersity index of 0.244 were recorded for the loaded Lim-SLNs, as compared to 203 ± 1.5 nm (PI 0.213) for the non-loaded (blank) SLNs. The loading of the monoterpene derivative into glycerol monostearate SLNs fitted into the zero-order kinetics, and ameliorated both lipid peroxidation and cytotoxicity in a keratinocyte cell line. A promising formulation for antioxidant and anti-tumoral activities is here proposed. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico | pt |
dc.relation | CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) | pt |
dc.relation | FAPITEC/SE (Fundação de Apoio à Pesquisa e Inovação Tecnológica do Estado de Sergipe) | pt |
dc.relation | M-ERA-NET-0004/2015-PAIRED | pt |
dc.relation | UIDB/04469/2020 | pt |
dc.relation | PEst-OE/UID/AGR/04033/2019 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | (+)-limonene 1 | pt |
dc.subject | 2-epoxide | pt |
dc.subject | monoterpene | pt |
dc.subject | Imwitor 900K-SLN | pt |
dc.subject | lipid peroxidation | pt |
dc.subject | cytotoxicity | pt |
dc.subject | HaCaT cell line | pt |
dc.subject.mesh | Cell Line | pt |
dc.subject.mesh | Delayed-Action Preparations | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Keratinocytes | pt |
dc.subject.mesh | Lipid Peroxidation | pt |
dc.subject.mesh | Nanoparticles | pt |
dc.subject.mesh | Antioxidants | pt |
dc.subject.mesh | Cyclohexane Monoterpenes | pt |
dc.subject.mesh | Monoglycerides | pt |
dc.subject.mesh | Poloxamer | pt |
dc.title | (+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line | pt |
dc.type | article | - |
degois.publication.firstPage | 1449 | pt |
degois.publication.issue | 4 | pt |
degois.publication.title | International Journal of Molecular Sciences | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ijms21041449 | pt |
degois.publication.volume | 21 | pt |
dc.date.embargo | 2020-02-20 | * |
uc.date.periodoEmbargo | 0 | pt |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
crisitem.author.orcid | 0000-0002-9737-6017 | - |
crisitem.author.orcid | 0000-0003-2603-1377 | - |
crisitem.author.orcid | 0000-0001-6527-6612 | - |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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limonene-12epoxideloaded-slns-Evaluation-of-drug-release-antioxidant-activity-and-cytotoxicity-in-an-HaCaT-cell-lineInternational-Journal-of-Molecular-Sciences.pdf | 1.19 MB | Adobe PDF | View/Open |
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