Please use this identifier to cite or link to this item:
DC FieldValueLanguage
dc.contributor.authorPalma, Mariana-
dc.contributor.authorTrenkner, Lauren H.-
dc.contributor.authorRito, João-
dc.contributor.authorTavares, Ludgero C.-
dc.contributor.authorSilva, Emanuel-
dc.contributor.authorGlencross, Brett D.-
dc.contributor.authorJones, John Griffith-
dc.contributor.authorWade, Nicholas M.-
dc.contributor.authorViegas, Ivan-
dc.description.abstractPractical diets for commercial barramundi production rarely contain greater than 10% starch, used mainly as a binding agent during extrusion. Alternative ingredients such as digestible starch have shown some capacity to spare dietary protein catabolism to generate glucose. In the present study, a carnivorous fish species, the Asian seabass (Lates calcarifer) was subjected to two diets with the same digestible energy: Protein (P) - with high protein content (no digestible starch); and Starch (S) - with high digestible (pregelatinized) starch content. The effects of a high starch content diet on hepatic glycogen synthesis as well as the muscle and liver metabolome were studied using a complementary approach of 1H and 2H NMR. The hepatosomatic index was lower for fish fed high starch content diet while the concentration of hepatic glycogen was similar between groups. However, increased glycogen synthesis via the direct pathway was observed in the fish fed high starch content diet which is indicative of increased carbohydrate utilization. Multivariate analysis also showed differences between groups in the metabolome of both tissues. Univariate analysis revealed more variations in liver than in muscle of fish fed high starch content diet. Variations in metabolome were generally in agreement with the increase in the glycogen synthesis through direct pathway, however, this metabolic shift seemed to be insufficient to keep the growth rate as ensured by the diet with high protein content. Although liver glycogen does not make up a substantial quantity of total stored dietary energy in carnivorous fish, it is a key regulatory intermediate in dietary energy
dc.description.sponsorshipThis work was supported by the University of Queensland Research Scholarship (UQRS) and CSIRO postgraduate top-up scholarship programs, a grant from the Australian Centre for International Agricultural Research (ACIAR) project FIS-2006- 141, and co-funded by CSIRO Agriculture and Food. This work was also supported by Fundação para a Ciência e Tecnologia (FCT; Portugal) through National Funds with co-funding from ERDF/FEDER, within the PT2020 Partnership Agreement, and COMPETE 2020: individual grant to MP through Centro2020 (ReNature; Centro-01-0145-FEDER-000007); structural funds to Centre for Functional Ecology (UID/BIA/04004/2013 and UID/BIA/04004/2019)-
dc.publisherFrontiers Media
dc.subjectAsian seabasspt
dc.subject2H NMRpt
dc.subjecthepatic glycogenpt
dc.titleLimitations to Starch Utilization in Barramundi (Lates calcarifer) as Revealed by NMR-Based Metabolomicspt
degois.publication.titleFrontiers in Physiologypt
item.fulltextCom Texto completo-
item.grantfulltextopen- - Centre for Functional Ecology - Science for People & the Planet-
Appears in Collections:I&D CFE - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
Show simple item record

Page view(s)

checked on Nov 28, 2023


checked on Nov 28, 2023

Google ScholarTM




This item is licensed under a Creative Commons License Creative Commons