Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/106094
Title: α-l-Fucosidases from Bursaphelenchus xylophilus Secretome—Molecular Characterization and Their Possible Role in Breaking Down Plant Cell Walls
Authors: Cardoso, Joana M. S. 
Fonseca, Luís 
Abrantes, Isabel 
Keywords: cell wall; fucosidase; pine wood nematode; pine trees; secretome
Issue Date: 2020
Publisher: MDPI
Project: POINTERSPTDC /ASP-SIL/31999/2017 (POCI-01-145-FEDER-031999) 
UID/BIA/04004/2019 
Project ReNATURE—Valorization of theNatural Endogenous Resources of the Centro Region (Centro 2020, Centro-01-0145-FEDER-000007) 
Instituto do Ambiente, Tecnologia e Vida 
Serial title, monograph or event: Forests
Volume: 11
Issue: 3
Abstract: The pinewood nematode (PWN) Bursaphelenchus xylophilus, the causal agent of the pine wilt disease (PWD), enters above-ground parts of the tree, migrates through the resin canals and feeds on plant cells causing extensive damage. In order to penetrate the cell wall and establish a parasitic relationshipwith host trees, the PWNneeds to secreteamixture of active cellwall degrading enzymes. In maritime pine, Pinus pinaster, which is high susceptible to PWN, xyloglucan is the major hemicellulosic polysaccharide in primary cells. The xyloglucan backbone is susceptible to hydrolysis by numerous endoglucanases, some of them specific to xyloglucan. However, to completely degrade xyloglucan, all substitutions on the glucan backbones must be released, and l-fucose residues in xyloglucan branches are released by -l-fucosidases. In the present study, the molecular characterization of two -l-fucosidases found in PWN secretome was performed. Moreover, a novel -l-fucosidase was identified and its cDNAand gene sequencewere determined. The three-dimensional structures of these -l-fucosidases were predicted and the transcript levels were analyzed, thus providing new insights into fundamental PWN biology and the possible role of these proteins as cell wall degrading enzymes.
URI: https://hdl.handle.net/10316/106094
ISSN: 1999-4907
DOI: 10.3390/f11030265
Rights: openAccess
Appears in Collections:I&D CFE - Artigos em Revistas Internacionais

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