Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/105368
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dc.contributor.authorGouveia, Marcos-
dc.contributor.authorSorčan, Tjaša-
dc.contributor.authorZemljič-Jokhadar, Špela-
dc.contributor.authorTravasso, Rui D.M.-
dc.contributor.authorLiović, Mirjana-
dc.date.accessioned2023-02-20T11:46:09Z-
dc.date.available2023-02-20T11:46:09Z-
dc.date.issued2021-
dc.identifier.issn1932-6203-
dc.identifier.urihttps://hdl.handle.net/10316/105368-
dc.description.abstractWe examined keratin aggregate formation and the possible mechanisms involved. With this aim, we observed the effect that different ratios between mutant and wild-type keratins expressed in cultured keratinocytes may have on aggregate formation in vitro, as well as how keratin aggregate formation affects the mechanical properties of cells at the cell cortex. To this end we prepared clones with expression rates as close as possible to 25%, 50% and 100% of the EGFP-K14 proteins (either WT or R125P and V270M mutants). Our results showed that only in the case of the 25% EGFP-K14 R125P mutant significant differences could be seen. Namely, we observed in this case the largest accumulation of keratin aggregates and a significant reduction in cell stiffness. To gain insight into the possible mechanisms behind this observation, we extended our previous mathematical model of keratin dynamics by implementing a more complex reaction network that considers the coexistence of wild-type and mutant keratins in the cell. The new model, consisting of a set of coupled, non-linear, ordinary differential equations, allowed us to draw conclusions regarding the relative amounts of intermediate filaments and aggregates in cells, and suggested that aggregate formation by asymmetric binding between wild-type and mutant keratins could explain the data obtained on cells grown in culture.pt
dc.language.isoengpt
dc.publisherPublic Library of Sciencept
dc.relationSFRH/BD/136046/ 2018pt
dc.relationUIDB/04564/ 2020pt
dc.relationUIDP/04564/2020pt
dc.relationCELSA Alliance and ARRS J3-3078 grants to M.L., the Slovenian Research Agency Program Grant P1- 0390, and the Republic of Slovenia Ministry of Education, Science and Sport and the EraCoSysMed (JTC-2 2017) “4D-HEALING” grant”pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.titleA mathematical model for the dependence of keratin aggregate formation on the quantity of mutant keratin expressed in EGFP-K14 R125P keratinocytespt
dc.typearticlept
degois.publication.firstPagee0261227pt
degois.publication.issue12pt
degois.publication.titlePLoS ONEpt
dc.peerreviewedyespt
dc.identifier.doi10.1371/journal.pone.0261227-
degois.publication.volume16pt
dc.date.embargo2021-01-01*
dc.identifier.pmid34962936-
uc.date.periodoEmbargo0pt
dc.identifier.eissn1932-6203-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypearticle-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.cerifentitytypePublications-
crisitem.author.orcid0000-0001-6078-0721-
Appears in Collections:I&D CFis - Artigos em Revistas Internacionais
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This item is licensed under a Creative Commons License Creative Commons