Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/105277
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Lopes, Ralf | - |
dc.contributor.author | Fuentes-Castillo, Danny | - |
dc.contributor.author | Fontana, Herrison | - |
dc.contributor.author | Rodrigues, Larissa | - |
dc.contributor.author | Dantas, Karine | - |
dc.contributor.author | Cerdeira, Louise | - |
dc.contributor.author | Henriques, Isabel | - |
dc.contributor.author | Lincopan, Nilton | - |
dc.date.accessioned | 2023-02-14T11:14:32Z | - |
dc.date.available | 2023-02-14T11:14:32Z | - |
dc.date.issued | 2021-02-09 | - |
dc.identifier.issn | 2379-5077 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/105277 | - |
dc.description.abstract | The global spread of antibiotic-resistant bacteria and their resistance genes is a critical issue that is no longer restricted to hospital settings, but also represents a growing problem involving environmental and food safety. In this study, we have performed a microbiological and genomic investigation of critical priority pathogens resistant to broad-spectrum cephalosporins and showing endophytic lifestyles in fresh vegetables sold in a country with high endemicity of extended-spectrum β-lactamases (ESBLs). We report the isolation of international high-risk clones of CTX-M-15-producing Escherichia coli, belonging to clonal complexes CC38 and CC648, and Klebsiella pneumoniae of complex CC307 from macerated tissue of surface-sterilized leaves of spinach, cabbage, arugula, and lettuce. Regardless of species, all ESBL-positive isolates were able to endophytically colonize common bean (Phaseolus vulgaris) seedlings, showed resistance to acid pH, and had a multidrug-resistant (MDR) profile to clinically relevant antibiotics (i.e., broad-spectrum cephalosporins, aminoglycosides, and fluoroquinolones). Genomic analysis of CTX-M-producing endophytic Enterobacterales revealed a wide resistome (antibiotics, biocides, disinfectants, and pesticides) and virulome, and genes for endophytic fitness and for withstanding acidic conditions. Transferable IncFIB and IncHI2A plasmids carried bla CTX-M-15 genes and, additionally, an IncFIB plasmid (named pKP301cro) also harbored genes encoding resistance to heavy metals. These data support the hypothesis that fresh vegetables marketed for consumption can act as a figurative Trojan horse for the hidden spread of international clones of critical WHO priority pathogens producing ESBLs, and/or their resistance genes, to humans and other animals, which is a critical issue within a food safety and broader public and environmental health perspective.IMPORTANCE Extended-spectrum β-lactamases (ESBL)-producing Enterobacterales are a leading cause of human and animal infections, being classified as critical priority pathogens by the World Health Organization. Epidemiological studies have shown that spread of ESBL-producing bacteria is not a problem restricted to hospitals, but also represents a growing problem involving environmental and food safety. In this regard, CTX-M-type β-lactamases have become the most widely distributed and clinically relevant ESBLs worldwide. Here, we have investigated the occurrence and genomic features of ESBL-producing Enterobacterales in surface-sterilized fresh vegetables. We have uncovered that international high-risk clones of CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae harboring a wide resistome and virulome, carry additional genes for endophytic fitness and resistance to acidic conditions. Furthermore, we have demonstrated that these CTX-M-15-positive isolates are able to endophytically colonize plant tissues. Therefore, we believe that fresh vegetables can act as a figurative Trojan horse for the hidden spread of critical priority pathogens exhibiting endophytic lifestyles. | pt |
dc.language.iso | eng | pt |
dc.publisher | American Society for Microbiology | pt |
dc.relation | Bill & Melinda Gates Foundation (Grand Challenges Explorations Brazil—new approaches to characterize the global burden of antimicrobial resistance, grant OPP1193112 | pt |
dc.relation | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, grants 2016/08593-9 and 15/21325-0 | pt |
dc.relation | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq Grants 443819/ 2018-1, 312249/2017-9 and 433128/2018-6) | pt |
dc.relation | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, grant 1541491/2015-8, finance code 001) | pt |
dc.relation | UIDP/50017/2020 | pt |
dc.relation | UIDB/50017/ 2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | E. coli ST648 | pt |
dc.subject | E. coli ST38 | pt |
dc.subject | K. pneumoniae CC307 | pt |
dc.subject | CTX-M-15 | pt |
dc.subject | food | pt |
dc.subject | One Health | pt |
dc.subject | ESBL | pt |
dc.title | Endophytic Lifestyle of Global Clones of Extended-Spectrum β-Lactamase-Producing Priority Pathogens in Fresh Vegetables: a Trojan Horse Strategy Favoring Human Colonization? | pt |
dc.type | article | - |
degois.publication.issue | 1 | pt |
degois.publication.title | mSystems | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1128/mSystems.01125-20 | pt |
degois.publication.volume | 6 | pt |
dc.date.embargo | 2021-02-09 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairetype | article | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.orcid | 0000-0001-7717-4939 | - |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Endophytic Lifestyle of Global Clones of Extended-Spectrum.pdf | 5.73 MB | Adobe PDF | Ver/Abrir |
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