Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/10523
Title: | Surfactant−DNA Gel Particles: Formation and Release Characteristics | Authors: | Morán, M. Carmen Miguel, M. Graça Lindman, Björn |
Issue Date: | 10-Dec-2007 | Publisher: | American Chemical Society | Citation: | Biomacromolecules. 8:12 (2007) 3886-3892 | Abstract: | Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report on the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant cetyltrimetrylammonium bromide (CTAB). By using CTAB, the formation of DNA reservoir gel particles, without adding any kind of cross-linker or organic solvent, has been demonstrated. Particles have been characterized with respect to the degree of DNA entrapment, surface morphology, and secondary structure of DNA in the particles. The swelling/deswelling behavior and the DNA release have been investigated in response to salt additions. Analysis of the data has suggested a higher degree of interaction between ssDNA and the cationic surfactant, confirming the stronger amphiphilic character of the denatured DNA. Fluorescence microscopy studies have suggested that the formation of these particles is associated with a conservation of the secondary structure of DNA. | URI: | https://hdl.handle.net/10316/10523 | ISSN: | 1525-7797 | DOI: | 10.1021/bm700850z | Rights: | openAccess |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Surfactant-DNA Gel Particles.pdf | 840.79 kB | Adobe PDF | View/Open |
SCOPUSTM
Citations
40
checked on Oct 14, 2024
WEB OF SCIENCETM
Citations
5
37
checked on Oct 2, 2024
Page view(s) 50
385
checked on Oct 15, 2024
Download(s)
332
checked on Oct 15, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.