Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/10523
Title: Surfactant−DNA Gel Particles: Formation and Release Characteristics
Authors: Morán, M. Carmen 
Miguel, M. Graça 
Lindman, Björn 
Issue Date: 10-Dec-2007
Publisher: American Chemical Society
Citation: Biomacromolecules. 8:12 (2007) 3886-3892
Abstract: Aqueous mixtures of oppositely charged polyelectrolytes undergo associative phase separation, resulting in coacervation, gelation, or precipitation. This phenomenon has been exploited here to form DNA gel particles by interfacial diffusion. We report on the formation of DNA gel particles by mixing solutions of DNA (either single-stranded (ssDNA) or double-stranded (dsDNA)) with solutions of cationic surfactant cetyltrimetrylammonium bromide (CTAB). By using CTAB, the formation of DNA reservoir gel particles, without adding any kind of cross-linker or organic solvent, has been demonstrated. Particles have been characterized with respect to the degree of DNA entrapment, surface morphology, and secondary structure of DNA in the particles. The swelling/deswelling behavior and the DNA release have been investigated in response to salt additions. Analysis of the data has suggested a higher degree of interaction between ssDNA and the cationic surfactant, confirming the stronger amphiphilic character of the denatured DNA. Fluorescence microscopy studies have suggested that the formation of these particles is associated with a conservation of the secondary structure of DNA.
URI: http://hdl.handle.net/10316/10523
ISSN: 1525-7797
DOI: 10.1021/bm700850z
Rights: openAccess
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
Surfactant-DNA Gel Particles.pdf840.79 kBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

33
checked on Feb 18, 2020

WEB OF SCIENCETM
Citations

30
checked on May 29, 2020

Page view(s)

234
checked on Aug 12, 2020

Download(s)

96
checked on Aug 12, 2020

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.