Utilize este identificador para referenciar este registo: https://hdl.handle.net/10316/103963
Título: Candida Extracellular Nucleotide Metabolism Promotes Neutrophils Extracellular Traps Escape
Autor: Afonso, Mariana
Mestre, Ana Rita 
Silva, Guilherme 
Almeida, Ana Catarina 
Cunha, Rodrigo A. 
Meyer-Fernandes, José Roberto
Gonçalves, Teresa 
Rodrigues, Lisa 
Palavras-chave: Candida,; neutrophils; nucleotide metabolism; nucleotidase; neutrophil extracellular traps escape
Data: 2021
Editora: Frontiers Media S.A.
Projeto: CENTRO-01-0246-FEDER-000010 
CENTRO-01-0145-FEDER-000012-HealthyAging2020 
POCI-01-0145-FEDER-007440 
UID/NEU/04539/2019 
Título da revista, periódico, livro ou evento: Frontiers in Cellular and Infection Microbiology
Volume: 11
Resumo: Host innate immunity is fundamental to the resistance against Candida albicans and Candida glabrata infection, two of the most important agents contributing to human fungal infections. Phagocytic cells, such as neutrophils, constitute the first line of host defense mechanisms, and the release of neutrophil extracellular traps (NETs) represent an important strategy to immobilize and to kill invading microorganisms, arresting the establishment of infection. The purinergic system operates an important role in the homeostasis of immunity and inflammation, and ectophosphatase and ectonucleotidase activities are recognized as essential for survival strategies and infectious potential of several pathogens. The expression and unique activity of a 3'-nucleotidase/nuclease (3'NT/NU), able to hydrolyze not only AMP but also nucleic acids, has been considered as part of a possible mechanism of microbes to escape from NETs. The aim of the present study was to evaluate if yeasts escape from the NET-mediated killing through their 3'NT/NU enzymatic activity contributing to NET-hydrolysis. After demonstrating the presence of 3'NT/NU activity in C. albicans, C. glabrata, and Saccharomyces cerevisiae, we show that, during neutrophils-Candida interaction, when NETs formation and release are triggered, NETs digestion occurs and this process of NETs disruption promoted by yeast cells was prevented by ammonium tetrathiomolybdate (TTM), a 3'NT/NU inhibitor. In conclusion, although the exact nature and specificity of yeasts ectonucleotidases are not completely unraveled, we highlight the importance of these enzymes in the context of infection, helping yeasts to overcome host defenses, whereby C. albicans and C. glabrata can escape NET-mediate killing through their 3'NT/NU activity.
URI: https://hdl.handle.net/10316/103963
ISSN: 2235-2988
DOI: 10.3389/fcimb.2021.678568
Direitos: openAccess
Aparece nas coleções:I&D CNC - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais

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