Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/103685
DC Field | Value | Language |
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dc.contributor.author | Bordalo, Mário | - |
dc.contributor.author | Seabra, Inês J. | - |
dc.contributor.author | Silva, Andreia Bento | - |
dc.contributor.author | Terrasso, Ana Paula | - |
dc.contributor.author | Brito, Catarina | - |
dc.contributor.author | Serra, Margarida | - |
dc.contributor.author | Bronze, Maria R. | - |
dc.contributor.author | Duarte, Catarina M. M. | - |
dc.contributor.author | Braga, Mara E. M. | - |
dc.contributor.author | de Sousa, Herminio C. | - |
dc.contributor.author | Serra, Ana Teresa | - |
dc.date.accessioned | 2022-11-21T11:36:26Z | - |
dc.date.available | 2022-11-21T11:36:26Z | - |
dc.date.issued | 2021-09-15 | - |
dc.identifier.issn | 2076-3921 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/103685 | - |
dc.description.abstract | Bravo de Esmolfe (BE) is a traditional Portuguese apple highly appreciated by consumers due to its peculiar flavor and aroma. This apple contains higher concentration of phenolic compounds than other cultivars and is thus considered a rich source of antioxidants. Its sensorial and functional properties have attracted farmers' associations to increase BE production. However, a large quantity of apples is wasted due to storage/transportation procedures that impact BE's quality attributes. In this work, we applied high-pressure extraction methodologies to generate antioxidant-rich fractions from BE residues aiming at adding high value to these agro-food by-products. We performed a first extraction step using supercritical CO2, followed by a second extraction step where different CO2 + ethanol mixtures (10-100% v/v) were tested. All experiments were carried out at 25 MPa and 50 °C. Extracts were characterized in terms of global yield, phenolic content and antioxidant activity using chemical (ORAC, HOSC, HORAC) and cell-based assays (CAA). We demonstrated that, although the pressurized 100% ethanol condition promoted the highest recovery of phenolic compounds (509 ± 8 mg GAE/100 g BE residues), the extract obtained with 40% ethanol presented the highest CAA (1.50 ± 0.24 µmol QE/g dw) and ORAC (285 ± 16 µmol TEAC/g dw), as well as HOSC and HORAC values, which correlated with its content of epicatechin and procyanidin B2. Noteworthy, this fraction inhibited free radical production in human neurospheroids derived from NT2 cells, a robust 3D cell model for neuroprotective testing. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | iNOVA4Health—UIDB/04462/2020 | pt |
dc.relation | iNOVA4Health—UIDP/04462/2020 | pt |
dc.relation | CIEPQPF (PEst-C/EQB/UI0102/2019 andUIDB/00102/2020) | pt |
dc.relation | INTERFACE Program, through the Innovation, Technology and Circular Economy Fund (FITEC) | pt |
dc.relation | Individual Grant CEECIND/04801/2017 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | Bravo de Esmolfe | pt |
dc.subject | apple | pt |
dc.subject | antioxidants | pt |
dc.subject | CO2 extraction | pt |
dc.subject | phenolic compounds | pt |
dc.subject | Caco-2 cells | pt |
dc.subject | neurospheroids | pt |
dc.title | Using High-Pressure Technology to Develop Antioxidant-Rich Extracts from Bravo de Esmolfe Apple Residues | pt |
dc.type | article | - |
degois.publication.firstPage | 1469 | pt |
degois.publication.issue | 9 | pt |
degois.publication.title | Antioxidants | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/antiox10091469 | pt |
degois.publication.volume | 10 | pt |
dc.date.embargo | 2021-09-15 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
crisitem.author.researchunit | CIEPQPF – Chemical Process Engineering and Forest Products Research Centre | - |
crisitem.author.researchunit | CIEPQPF – Chemical Process Engineering and Forest Products Research Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0003-4142-4021 | - |
crisitem.author.orcid | 0000-0002-2629-7805 | - |
Appears in Collections: | I&D CIEPQPF - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Using-highpressure-technology-to-develop-antioxidantrich-extracts-from-bravo-de-esmolfe-apple-residuesAntioxidants.pdf | 912.46 kB | Adobe PDF | View/Open |
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