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Title: KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds
Authors: Teixeira, Pedro
Pinto, Nuno 
Henriques, Isabel 
Tacão, Marta 
Keywords: antibiotic resistance; carbapenemases; Enterobacterales; urban aquatic environments
Issue Date: 2022
Project: FCT/MCTES through national funds to CESAM (UIDP/50017/2020+UIDB/50017/2020 + LA/P/0094/2020), CFE (UIDB/04004/2020), and individual grants to P.T. (SFRH/BD/132046/2017) and M.T. (CEECIND/00977/2020). 
Serial title, monograph or event: International Journal of Environmental Research and Public Health
Volume: 19
Issue: 10
Abstract: Carbapenems are antibiotics of pivotal importance in human medicine, the efficacy of which is threatened by the increasing prevalence of carbapenem-resistant Enterobacterales (CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban ponds over a one-year period and retrieved 23 isolates. These were submitted to BOX-PCR, PFGE, 16S rDNA sequencing, antibiotic susceptibility tests, detection of carbapenemase-encoding genes, and conjugation assays. Isolates were affiliated with Klebsiella (n = 1), Raoultella (n = 11), Citrobacter (n = 8), and Enterobacter (n = 3). Carbapenemase-encoding genes were detected in 21 isolates: blaKPC (n = 20), blaGES-5 (n = 6), and blaVIM (n = 1), with 7 isolates carrying two carbapenemase genes. Clonal isolates were collected from different ponds and in different campaigns. Citrobacter F6, Raoultella N9, and Enterobacter N10 were predicted as pathogens from whole-genome sequence analysis, which also revealed the presence of several resistance genes and mobile genetic elements. We found that blaKPC-3 was located on Tn4401b (Citrobacter F6 and Enterobacter N10) or Tn4401d (Raoultella N9). The former was part of an IncFIA-FII pBK30683-like plasmid. In addition, blaGES-5 was in a class 3 integron, either chromosomal (Raoultella N9) or plasmidic (Enterobacter N10). Our findings confirmed the role of urban ponds as reservoirs and dispersal sites for CRE.
ISSN: 1660-4601
DOI: 10.3390/ijerph19105848
Rights: openAccess
Appears in Collections:I&D CFE - Artigos em Revistas Internacionais

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