Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/103302
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Teixeira, Pedro | - |
dc.contributor.author | Pinto, Nuno | - |
dc.contributor.author | Henriques, Isabel | - |
dc.contributor.author | Tacão, Marta | - |
dc.date.accessioned | 2022-11-03T09:54:46Z | - |
dc.date.available | 2022-11-03T09:54:46Z | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 1660-4601 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/103302 | - |
dc.description.abstract | Carbapenems are antibiotics of pivotal importance in human medicine, the efficacy of which is threatened by the increasing prevalence of carbapenem-resistant Enterobacterales (CRE). Urban ponds may be reservoirs of CRE, although this hypothesis has been poorly explored. We assessed the proportion of CRE in urban ponds over a one-year period and retrieved 23 isolates. These were submitted to BOX-PCR, PFGE, 16S rDNA sequencing, antibiotic susceptibility tests, detection of carbapenemase-encoding genes, and conjugation assays. Isolates were affiliated with Klebsiella (n = 1), Raoultella (n = 11), Citrobacter (n = 8), and Enterobacter (n = 3). Carbapenemase-encoding genes were detected in 21 isolates: blaKPC (n = 20), blaGES-5 (n = 6), and blaVIM (n = 1), with 7 isolates carrying two carbapenemase genes. Clonal isolates were collected from different ponds and in different campaigns. Citrobacter F6, Raoultella N9, and Enterobacter N10 were predicted as pathogens from whole-genome sequence analysis, which also revealed the presence of several resistance genes and mobile genetic elements. We found that blaKPC-3 was located on Tn4401b (Citrobacter F6 and Enterobacter N10) or Tn4401d (Raoultella N9). The former was part of an IncFIA-FII pBK30683-like plasmid. In addition, blaGES-5 was in a class 3 integron, either chromosomal (Raoultella N9) or plasmidic (Enterobacter N10). Our findings confirmed the role of urban ponds as reservoirs and dispersal sites for CRE. | pt |
dc.language.iso | eng | pt |
dc.relation | UIDP/50017/2020 | pt |
dc.relation | UIDB/50017/2020 | pt |
dc.relation | LA/P/0094/2020 | pt |
dc.relation | CFE-Centre for Functional Ecology-Science for People & the Planet’s Strategic Plan (UIDB/04004/2020) | pt |
dc.relation | SFRH/BD/132046/2017 | pt |
dc.relation | CEECIND/00977/2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | antibiotic resistance | pt |
dc.subject | carbapenemases | pt |
dc.subject | Enterobacterales | pt |
dc.subject | urban aquatic environments | pt |
dc.subject.mesh | Carbapenems | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Klebsiella | pt |
dc.subject.mesh | Microbial Sensitivity Tests | pt |
dc.subject.mesh | Ponds | pt |
dc.subject.mesh | Enterobacteriaceae Infections | pt |
dc.title | KPC-3-, GES-5-, and VIM-1-Producing Enterobacterales Isolated from Urban Ponds | pt |
dc.type | article | - |
degois.publication.firstPage | 5848 | pt |
degois.publication.issue | 10 | pt |
degois.publication.title | International Journal of Environmental Research and Public Health | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ijerph19105848 | pt |
degois.publication.volume | 19 | pt |
dc.date.embargo | 2022-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairetype | article | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Centre for Environmental and Marine Studies - CESAM | - |
crisitem.project.grantno | Centre for Functional Ecology - Science for People & the Planet | - |
crisitem.author.orcid | 0000-0001-7717-4939 | - |
crisitem.author.orcid | 0000-0001-7965-4928 | - |
Appears in Collections: | I&D CFE - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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KPC3-GES5-and-VIM1Producing-Enterobacterales-Isolated-from-Urban-PondsInternational-Journal-of-Environmental-Research-and-Public-Health.pdf | 1.66 MB | Adobe PDF | View/Open |
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