Please use this identifier to cite or link to this item:
Title: Effect of short-chain primary alcohols on fluidity and activity of sarcoplasmic reticulum membranes
Authors: Almeida, Leonor M. 
Vaz, Winchil L. C. 
Stuempel, Juergen 
Madeira, Vítor M. C. 
Issue Date: Aug-1986
Publisher: American Chemical Society
Citation: Biochemistry, 25:17 (1986) 4832-4839
Abstract: Intramolecular excimer formation with the fluorescent probe 1,3-di( 1 -pyrenyl)propane, differential scanning calorimetry, and X-ray diffraction were used to assess the effect of ethanol, 1-butanol, and 1-hexanol on the bilayer organization in model membranes, sarcoplasmic reticulum (SR) lipids and native SR membranes. These alcohols have fluidizing effects on membranes and lower the main transition temperature of dimyristoylphosphatidylcholine (DMPC), but only 1-hexanol alters the cooperativity of the phase transition and significantly increases the thickness of DMPC bilayers. The interaction of the three alcohols with the SR Ca2+ pump was also investigated. Hydrolysis of ATP and coupled Ca2+ uptake are differently sensitive to the three alcohols. Whereas ethanol and I-butanol inhibited the Ca2+ uptake, I-hexanol stimulated it. Nevertheless, the energetic efficiency of the pump (Ca2+/ATP) is not significantly affected by ethanol or 1-hexanol, but uncoupling was observed with 1-butanol at high concentrations. The different effects of alcohols on the activity of SR membranes rule out an unitary mechanism of action on the basis of fluidity changes induced in the lipid bilayer. Depending on the chain length, the alcohols interact with the SR membranes in different domains, perturbing differently the Ca2+-pump activity.
ISSN: 0006-2960
DOI: 10.1021/bi00365a017
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
Effect of Short-Chain Primary Alcohols on Fluidity.pdf1.61 MBAdobe PDFView/Open
Show full item record


checked on Feb 12, 2024

Citations 5

checked on May 2, 2023

Page view(s) 50

checked on Feb 20, 2024


checked on Feb 20, 2024

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.