Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/10296
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dc.contributor.authorAlmeida, Leonor M.-
dc.contributor.authorVaz, Winchil L. C.-
dc.contributor.authorStuempel, Juergen-
dc.contributor.authorMadeira, Vítor M. C.-
dc.date.accessioned2009-06-24T10:42:53Z-
dc.date.available2009-06-24T10:42:53Z-
dc.date.issued1986-08-
dc.identifier.citationBiochemistry, 25:17 (1986) 4832-4839en_US
dc.identifier.issn0006-2960-
dc.identifier.urihttps://hdl.handle.net/10316/10296-
dc.description.abstractIntramolecular excimer formation with the fluorescent probe 1,3-di( 1 -pyrenyl)propane, differential scanning calorimetry, and X-ray diffraction were used to assess the effect of ethanol, 1-butanol, and 1-hexanol on the bilayer organization in model membranes, sarcoplasmic reticulum (SR) lipids and native SR membranes. These alcohols have fluidizing effects on membranes and lower the main transition temperature of dimyristoylphosphatidylcholine (DMPC), but only 1-hexanol alters the cooperativity of the phase transition and significantly increases the thickness of DMPC bilayers. The interaction of the three alcohols with the SR Ca2+ pump was also investigated. Hydrolysis of ATP and coupled Ca2+ uptake are differently sensitive to the three alcohols. Whereas ethanol and I-butanol inhibited the Ca2+ uptake, I-hexanol stimulated it. Nevertheless, the energetic efficiency of the pump (Ca2+/ATP) is not significantly affected by ethanol or 1-hexanol, but uncoupling was observed with 1-butanol at high concentrations. The different effects of alcohols on the activity of SR membranes rule out an unitary mechanism of action on the basis of fluidity changes induced in the lipid bilayer. Depending on the chain length, the alcohols interact with the SR membranes in different domains, perturbing differently the Ca2+-pump activity.en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsopenAccesseng
dc.titleEffect of short-chain primary alcohols on fluidity and activity of sarcoplasmic reticulum membranesen_US
dc.typearticleen_US
dc.identifier.doi10.1021/bi00365a017-
uc.controloAutoridadeSim-
item.fulltextCom Texto completo-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.languageiso639-1en-
item.openairetypearticle-
item.cerifentitytypePublications-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0002-7769-4712-
crisitem.author.orcid0000-0002-3797-4330-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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