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Title: | UHPLC-ToF-MS method for determination of multi-mycotoxins in maize: Development and validation | Authors: | Silva, Ana Sanches Brites, Carla Pouca, Ana Vila Barbosa, Jorge Freitas, Andreia |
Keywords: | Cereals; Maize; Mycotoxins; Time-of-flight mass spectrometry; Ultra-high performance liquid chromatography; Validation | Issue Date: | Nov-2019 | Project: | Rural Development Program through the Operational Group QUALIMILHO- New sustainable integration strategies that guarantee quality and safety in the national maize, PDR2020 nº 101–031295 (2017–2020) | Serial title, monograph or event: | Current Research in Food Science | Volume: | 1 | Abstract: | An Ultra-High Performance Liquid Chromatography combined with Time-of-Flight Mass Spectrometry (UHPLC-ToF-MS) method has been developed for determination of nine mycotoxins, namely aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZEA), toxin T2 (T2) and fumonisins (FB1 and FB2) in maize. The method included a two-step extraction with acetonitrile 80% (v/v). After optimization, the analytical method was validated. The different concentrations tested take in account the Maximum Levels (ML) for maize (Commission Regulation EC no. 1881/2006) and good results for repeatability (%RSDr ≤ 15.4%), reproducibility (%RSDR ≤ 15.9%) and recovery (77.8-110.4%, except for AFG2 at 2 μg/kg which presented a recovery of 73.4%) were achieved. These met the performance criteria imposed by Commission Regulation (EC) no. 401/2006. The method was applied to twenty-two samples from Portuguese producers of maize. Fumonisins were the most frequently detected mycotoxins, but the levels do not exceed those imposed by European legislation. | URI: | https://hdl.handle.net/10316/101587 | ISSN: | 26659271 | DOI: | 10.1016/j.crfs.2019.07.001 | Rights: | openAccess |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais |
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