Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/101587
Title: UHPLC-ToF-MS method for determination of multi-mycotoxins in maize: Development and validation
Authors: Silva, Ana Sanches 
Brites, Carla
Pouca, Ana Vila
Barbosa, Jorge 
Freitas, Andreia 
Keywords: Cereals; Maize; Mycotoxins; Time-of-flight mass spectrometry; Ultra-high performance liquid chromatography; Validation
Issue Date: Nov-2019
Project: Rural Development Program through the Operational Group QUALIMILHO- New sustainable integration strategies that guarantee quality and safety in the national maize, PDR2020 nº 101–031295 (2017–2020) 
metadata.degois.publication.title: Current Research in Food Science
metadata.degois.publication.volume: 1
Abstract: An Ultra-High Performance Liquid Chromatography combined with Time-of-Flight Mass Spectrometry (UHPLC-ToF-MS) method has been developed for determination of nine mycotoxins, namely aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZEA), toxin T2 (T2) and fumonisins (FB1 and FB2) in maize. The method included a two-step extraction with acetonitrile 80% (v/v). After optimization, the analytical method was validated. The different concentrations tested take in account the Maximum Levels (ML) for maize (Commission Regulation EC no. 1881/2006) and good results for repeatability (%RSDr ≤ 15.4%), reproducibility (%RSDR ≤ 15.9%) and recovery (77.8-110.4%, except for AFG2 at 2 μg/kg which presented a recovery of 73.4%) were achieved. These met the performance criteria imposed by Commission Regulation (EC) no. 401/2006. The method was applied to twenty-two samples from Portuguese producers of maize. Fumonisins were the most frequently detected mycotoxins, but the levels do not exceed those imposed by European legislation.
URI: https://hdl.handle.net/10316/101587
ISSN: 26659271
DOI: 10.1016/j.crfs.2019.07.001
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais

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