EXPL/NEU- NMC/0541/2012

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Project title
EXPL/NEU- NMC/0541/2012
Code
RiboLoss Mechanism underlying ribosomal regulation in axonal development
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Abstract
Neurons present a defined structure, in which axons have a huge relevance in cellular communication. Increasing evidences point local protein translation as an important mechanism in several stages of axonal development. These include growth cone collapse, axonal pathfinding, retrograde survival and synaptic plasticity. For example, in axonal outgrowth there is a need for a structural reorganisation and newly synthesised proteins. Ribosomes, messenger-RNA (mRNA) or simply axonal transport are key examples of the machinery required for axonal development. Using neuronal models from peripheral nervous system (spinal motoneurons (MN), ciliary ganglia (CG) motoneurons) and central nervous system (hippocampal neurons), we were able to detect S6 and P0 ribosomal proteins by immunofluorescence, indicating the presence of ribosomes in early developmental stages. Using different technical approaches based on microscopy, such as quantitative immunofluorescence, FISH or live-cell imaging, will plan to explore our preliminary data and uncover the mechanisms that control ribosome and mRNA regulation in neuronal development.
 

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Issue DateTitleAuthor(s)TypeAccess
12014Activation of microglia bolsters synapse formationCristovão, Gonçalo ; Pinto, Maria J. ; Cunha, Rodrigo A. ; Almeida, Ramiro D. ; Gomes, Catarina A. articleopenAccess
22016Visualizing K48 Ubiquitination during Presynaptic Formation By Ubiquitination-Induced Fluorescence Complementation (UiFC)Pinto, Maria J. ; Pedro, Joana R. ; Costa, Rui O. ; Almeida, Ramiro D. articleopenAccess