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Title: | Olive Varieties under UV-B Stress Show Distinct Responses in Terms of Antioxidant Machinery and Isoform/Activity of RubisCO | Authors: | Piccini, Chiara Cai, Giampiero Dias, Maria Celeste Araújo, Márcia Parri, Sara Romi, Marco Faleri, Claudia Cantini, Claudio |
Keywords: | Antioxidant enzymes; Heat shock proteins; Olea europaea; RubisCO; Sucrose synthase; UV-B radiation | Issue Date: | 2021 | Project: | SFRH/BD/116801/2016 SFRH/BPD/100865/2014 |
Serial title, monograph or event: | International Journal of Molecular Sciences | Volume: | 22 | Issue: | 20 | Abstract: | In recent decades, atmospheric pollution led to a progressive reduction of the ozone layer with a consequent increase in UV-B radiation. Despite the high adaptation of olive trees to the Mediterranean environment, the progressive increase of UV-B radiation is a risk factor for olive tree cultivation. It is therefore necessary to understand how high levels of UV-B radiation affect olive plants and to identify olive varieties which are better adapted. In this study we analyzed two Italian olive varieties subjected to chronic UV-B stress. We focused on the effects of UV-B radiation on RubisCO, in terms of quantity, enzymatic activity and isoform composition. In addition, we also analyzed changes in the activity of antioxidant enzymes (SOD, CAT, GPox) to get a comprehensive picture of the antioxidant system. We also evaluated the effects of UV-B on the enzyme sucrose synthase. The overall damage at biochemical level was also assessed by analyzing changes in Hsp70, a protein triggered under stress conditions. The results of this work indicate that the varieties (Giar-raffa and Olivastra Seggianese) differ significantly in the use of specific antioxidant defense systems, as well as in the activity and isoform composition of RubisCO. Combined with a different use of sucrose synthase, the overall picture shows that Giarraffa optimized the use of GPox and opted for a targeted choice of RubisCO isoforms, in addition to managing the content of sucrose synthase, thereby saving energy during critical stress points. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | URI: | https://hdl.handle.net/10316/96286 | ISSN: | 1422-0067 | DOI: | 10.3390/ijms222011214 | Rights: | openAccess |
Appears in Collections: | I&D CFE - Artigos em Revistas Internacionais |
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ijms-22-11214-v3.pdf | 5.12 MB | Adobe PDF | View/Open |
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