Please use this identifier to cite or link to this item:
Title: Graft Protection Against Cold Ischemia Preservation: An Institute George Lopez 1 and Histidine-tryptophan-ketoglutarate Solution Appraisal
Authors: Panisello-Rosello, A
Castro-Benítez, C
Lopez, A
Balloji, S
Folch-Puy, E
Adam, R
Roselló-Catafau, J
Issue Date: Apr-2018
Project: Instituto de Salud Carlos III (ISCIII) through the FIS project PI12/0056 
Marie Sklodowska-Curie Grant Agreement No. 722619 (FOIE GRAS) 
Serial title, monograph or event: Transplantation Proceedings
Volume: 50
Issue: 3
Abstract: Cold storage of organs in preservation solutions, such as Institute George Lopez 1 (IGL-1) or histidine-tryptophan-ketoglutarate (HTK), is a mandatory step for organ transplantation. This preservation leads to an ischemic injury that affects the outcome of the organ. This article studies the liver graft eluate after organ recovery using IGL-1 or HTK solutions. We explore the influence of the volume used for washing out the liver and the consequences in the graft preservation when both solutions are used. Livers were washed out with different volumes of HTK and IGL-1 according to manufacturers' instructions and then preserved in both solutions for 24 hours at 4°C. Tissue and eluates were collected for subsequent analyses. We measured transaminases (aspartate aminotransferase and alanine aminotransferase), histology by hematoxylin/eosin staining, and red blood cell and hemoglobin counts, respectively. After washing out and cold storage, the IGL-1 processed livers showed better preservation than those with HTK solution; however, in this latter case, an important accumulation of erythrocytes was found when compared to IGL-1. These data were consistent with the higher hemoglobin and red blood cell counts observed for IGL-1 eluates after 24 hours. The volume used for washing out the organ depends on the composition and properties of the organ preservation solutions (ie, IGL-1 and HTK); this is an important factor for the graft cold preservation. The total volume used for washing out the graft should be considered because it has a direct impact on the total cost for clinical transplantations.
ISSN: 00411345
DOI: 10.1016/j.transproceed.2018.02.044
Rights: embargoedAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
TP2018.pdf320.28 kBAdobe PDFView/Open
Show full item record


checked on Dec 4, 2023


checked on Dec 2, 2023

Page view(s)

checked on Nov 28, 2023


checked on Nov 28, 2023

Google ScholarTM




This item is licensed under a Creative Commons License Creative Commons